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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: GC-MS analysis and in-vitro hypocholesterolemic, anti-rotavirus, anti-human colon carcinoma activities of the crude extract of a Japanese Ganoderma spp
Author(s): Elkhateeb, Waill A
Daba, Ghoson M
Sheir, Donia
El-Dein, Asmaa Negm
Fayad, Walid
Elmahdy, Elmahdy M
Shaheen, Mohamed N F
Thomas, Paul W
Wen, Ting-Chi
Keywords: biological activity
gas chromatography–mass spectrometry
human colon cancer
hypocholesterolemic activity
Issue Date: Jun-2019
Abstract: Background and objective Medicinal mushrooms are mines of various biologically active compounds. Therefore, chemical analysis and in-vitro evaluation of some biological activities of the Japanese originated mushroom Ganoderma spp. were conducted. Materials and methods Extraction of the fruiting bodies of Ganoderma spp. was accomplished using 80% methanol. This extract was investigated for its in-vitro cholesterol-lowering activity, anti-rotavirus effect, and anti-human colon cancer influence. Moreover, a gas chromatography–mass spectrometry analysis for this extract was performed. Results and conclusion The gas chromatography–mass spectrometry analysis resulted in the detection of 39 compounds, which were generally saturated and unsaturated fatty acids, and alkenes. The crude extract exhibited a promising in-vitro cholesterol-lowering activity (100±0%) after 96 h of incubation at room temperature. The same crude extract showed a moderate anti-rotavirus SA-11 strain effect with a therapeutic index of 9.3. Moreover, Ganoderma spp. extract displayed a strong activity toward HCT116 human colon carcinoma cell line, resulting in a cytotoxicity of 84.03±0.93% on HCT116 cell line monolayers. Ganoderma spp. crude extract represents a promising source of biologically active compounds that could by further investigations represent support and/or alternative to the currently used drugs.
DOI Link: 10.4103/epj.epj_50_18
Rights: This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License (, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
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