Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29826
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Survival and immune response of white shrimp Litopenaeus vannamei following single and concurrent infections with WSSV and Vibrio parahaemolyticus
Author(s): Pang, Huanying
Wang, Gang
Zhou, Shihui
Wang, Junlin
Zhao, Jichen
Hoare, Rowena
Monaghan, Sean J
Wang, Ziling
Sun, Chengbo
Contact Email: rowena.hoare1@stir.ac.uk
Keywords: Litopenaeus vannamei
Immune response
White spot syndrome virus (WSSV)
Vibrio parahaemolyticus
Co-infection
Issue Date: Sep-2019
Citation: Pang H, Wang G, Zhou S, Wang J, Zhao J, Hoare R, Monaghan SJ, Wang Z & Sun C (2019) Survival and immune response of white shrimp Litopenaeus vannamei following single and concurrent infections with WSSV and Vibrio parahaemolyticus. Fish and Shellfish Immunology, 92, pp. 712-718. https://doi.org/10.1016/j.fsi.2019.06.039
Abstract: The survival and immune responses of Litopenaeus vannamei were evaluated during white spot syndrome virus (WSSV) or Vibrio parahaemolyticus single and concurrent infections. The mortality, WSSV load, activities of 4 immune enzymes: acid phosphatase (ACP), alkaline phosphatase (AKP), peroxidase (POD) and superoxide dismutase (SOD), and the transcription of Evolutionarily Conserved Signaling Intermediate in Toll pathways of L.vannamei (LvECSIT) were quantified at 0, 3, 6, 12, 24, 48, 72 and 96 h post-infection (pi). The results showed: (i) the cumulative mortality of the co-infection group (WSSV and V. Parahaemolyticus 83%) was significantly lower than the WSSV infection group (97%) (P < 0.05) at 96 hpi; (ii) copies of WSSV in the co-infection group were significantly lower than that of the single infection group from 24 to 96 hpi (P < 0.05); (iii) ACP, AKP,POD and SOD activity in the gills of the co-infection group was higher than that of the WSSV group at12, 48 and 96 hpi (P < 0.05).The expression of LvECSIT mRNA in the co-infection group was significantly higher than in the WSSV infection group from 12 to 72 hpi (P < 0.05).The results indicate that proliferation of WSSV is inhibited by V.parahaemolyticus infection. In addition, infection with WSSV alone causes a significant reduction in some immune responses of shrimp than co-infection with WSSV and V.parahaemolyticus occurs at 26 °C. Third, LvECSIT, an essential member of TLR signaling pathway might play a crucial role in shrimp defense against WSSV – Vibrio co-infection.
DOI Link: 10.1016/j.fsi.2019.06.039
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Pang H, Wang G, Zhou S, Wang J, Zhao J, Hoare R, Monaghan SJ, Wang Z, Sun C (2019) Survival and immune response of white shrimp Litopenaeus vannamei following single and concurrent infections with WSSV and Vibrio parahaemolyticus, Fish and Shellfish Immunology, 92, pp. 712-718. DOI: https://doi.org/10.1016/j.fsi.2019.06.039. © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

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