Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29496
Appears in Collections:Aquaculture Conference Papers and Proceedings
Peer Review Status: Refereed
Author(s): Viegas, Ivan
Trenkner, Lauren H
Rito, João
Palma, Mariana
Tavares, Ludgero C
Jones, John G
Glencross, Brett D
Wade, Nicholas M
Contact Email: b.d.glencross@stir.ac.uk
Title: Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer)
Citation: Viegas I, Trenkner LH, Rito J, Palma M, Tavares LC, Jones JG, Glencross BD & Wade NM (2019) Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer). 13th International Congress on the Biology of Fish: Select papers from the Growth and Metabolism of Fishes and the Muscle Growth and Development symposia, Calgary, Alberta, Canada. Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, 231, pp. 170-176. https://doi.org/10.1016/j.cbpa.2019.02.025
Issue Date: May-2019
Conference Name: 13th International Congress on the Biology of Fish: Select papers from the Growth and Metabolism of Fishes and the Muscle Growth and Development symposia
Conference Location: Calgary, Alberta, Canada
Abstract: In aquaculture, there is high interest in substituting marine-derived with vegetable-based ingredients as energy source. Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both. In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O. Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT). Fractional synthetic rates for TAG-bound fatty acids and glycerol were quantified separately providing a detailed lipogenic profile. The FA profile differed substantially between muscle and VAT in both species, but their lipogenic fluxes revealed even greater differences. In European seabass, HS promoted DNL of TAG-bound FA, in muscle and VAT. High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling. In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected. VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling. This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.
Status: AM - Accepted Manuscript
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Viegas I, Trenkner LH, Rito J, Palma M, Tavares LC, Jones JG, Glencross BD & Wade NM (2019) Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer). Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, 231, pp. 170-176. DOI: https://doi.org/10.1016/j.cbpa.2019.02.025 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

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