Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29496
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dc.contributor.authorViegas, Ivanen_UK
dc.contributor.authorTrenkner, Lauren Hen_UK
dc.contributor.authorRito, Joãoen_UK
dc.contributor.authorPalma, Marianaen_UK
dc.contributor.authorTavares, Ludgero Cen_UK
dc.contributor.authorJones, John Gen_UK
dc.contributor.authorGlencross, Brett Den_UK
dc.contributor.authorWade, Nicholas Men_UK
dc.date.accessioned2019-05-15T00:02:06Z-
dc.date.available2019-05-15T00:02:06Z-
dc.date.issued2019-05en_UK
dc.identifier.urihttp://hdl.handle.net/1893/29496-
dc.description.abstractIn aquaculture, there is high interest in substituting marine-derived with vegetable-based ingredients as energy source. Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both. In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O. Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT). Fractional synthetic rates for TAG-bound fatty acids and glycerol were quantified separately providing a detailed lipogenic profile. The FA profile differed substantially between muscle and VAT in both species, but their lipogenic fluxes revealed even greater differences. In European seabass, HS promoted DNL of TAG-bound FA, in muscle and VAT. High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling. In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected. VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling. This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationViegas I, Trenkner LH, Rito J, Palma M, Tavares LC, Jones JG, Glencross BD & Wade NM (2019) Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer). 13th International Congress on the Biology of Fish: Select papers from the Growth and Metabolism of Fishes and the Muscle Growth and Development symposia, Calgary, Alberta, Canada. Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, 231, pp. 170-176. https://doi.org/10.1016/j.cbpa.2019.02.025en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Viegas I, Trenkner LH, Rito J, Palma M, Tavares LC, Jones JG, Glencross BD & Wade NM (2019) Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer). Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, 231, pp. 170-176. DOI: https://doi.org/10.1016/j.cbpa.2019.02.025 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectSeabassen_UK
dc.subjectBarramundien_UK
dc.subjectMuscleen_UK
dc.subjectVisceral adipose tissueen_UK
dc.subjectNMRen_UK
dc.subjectTriacylglycerolen_UK
dc.subjectFatty acidsen_UK
dc.subjectGlycerolen_UK
dc.titleImpact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer)en_UK
dc.typeConference Paperen_UK
dc.rights.embargodate2020-02-25en_UK
dc.rights.embargoreason[Viegas et al 2019 - Manuscript_CBPA_2018_189.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.cbpa.2019.02.025en_UK
dc.identifier.pmid30818019en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiologyen_UK
dc.citation.issn1095-6433en_UK
dc.citation.volume231en_UK
dc.citation.spage170en_UK
dc.citation.epage176en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderCommonwealth Scientific Industrial Research Organisationen_UK
dc.author.emailb.d.glencross@stir.ac.uken_UK
dc.citation.conferencelocationCalgary, Alberta, Canadaen_UK
dc.citation.conferencename13th International Congress on the Biology of Fish: Select papers from the Growth and Metabolism of Fishes and the Muscle Growth and Development symposiaen_UK
dc.citation.date25/02/2019en_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationUniversity of Queenslanden_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationCSIRO Agriculture and Fooden_UK
dc.identifier.scopusid2-s2.0-85062222565en_UK
dc.identifier.wtid1282185en_UK
dc.contributor.orcid0000-0003-1167-8530en_UK
dc.date.accepted2019-02-21en_UK
dc.description.refREF Compliant by Deposit in Stirling's Repositoryen_UK
dc.date.filedepositdate2019-05-14en_UK
Appears in Collections:Aquaculture Conference Papers and Proceedings

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