Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29478
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: MicroRNAs Involved in the Regulation of LC-PUFA Biosynthesis in Teleosts: miR-33 Enhances LC-PUFA Biosynthesis in Siganus canaliculatus by Targeting insig1 which in Turn Upregulates srebp1
Author(s): Sun, Jun Jun
Zheng, Li Guo
Chen, Cui Ying
Zhang, Jin Ying
You, Cui Hong
Zhang, Qing Hao
Ma, Hong Yu
Monroig, Oscar
Tocher, Douglas R
Wang, Shu Qi
Li, Yuan You
Contact Email: d.r.tocher@stir.ac.uk
Keywords: miR-33
insig1
srebp1
Δ6Δ5fads2
elovl5
LC-PUFA biosynthesis
Issue Date: Aug-2019
Citation: Sun JJ, Zheng LG, Chen CY, Zhang JY, You CH, Zhang QH, Ma HY, Monroig O, Tocher DR, Wang SQ & Li YY (2019) MicroRNAs Involved in the Regulation of LC-PUFA Biosynthesis in Teleosts: miR-33 Enhances LC-PUFA Biosynthesis in Siganus canaliculatus by Targeting insig1 which in Turn Upregulates srebp1. Marine Biotechnology, 21 (4), pp. 475-487. https://doi.org/10.1007/s10126-019-09895-w
Abstract: Post-transcriptional regulatory mechanisms play important roles in the regulation of LC-PUFA biosynthesis. Our previous study revealed that miR-33 could increase the expression of fatty acyl desaturases (fads2) in the rabbitfish Siganus canaliculatus, but the specific mechanism is unknown. Here, we confirmed that miR-33 could target the 3′UTR of insulin-induced gene 1 (insig1), resulting in downregulation of its protein level in the rabbitfish hepatocyte line (SCHL). In vitro overexpression of miR-33 inhibited the mRNA level of insig1 and increased the mRNA levels of Δ6Δ5 fads2 and elovl5, as well as srebp1. In SCHL cells, proteolytic activation of sterol-regulatory-element-binding protein-1 (Srebp1) was blocked by Insig1, with overexpression of insig1 decreasing mature Srebp1 level, while inhibition of insig1 led to the opposite effect. Srebp1 could enhance the promoter activity of Δ6Δ5 fads2 and elovl5, whose expression levels decreased with knockdown of srebp1 in SCHL. Overexpression of miR-33 also resulted in a higher conversion of 18:3n-3 to 18:4n-3 and 20:5n-3 to 22:5n-3, linked to desaturation and elongation via Δ6Δ5 Fads2 and Elovl5, respectively. The results suggested that the mechanism by which miR-33 regulates LC-PUFA biosynthesis in rabbitfish is through enhancing the expression of srebp1 by targeting insig1. The findings here provide more insight to the mechanism of miRNAs involvement in the regulation of LC-PUFA biosynthesis in teleosts.
DOI Link: 10.1007/s10126-019-09895-w
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: https://doi.org/10.1007/s10126-019-09895-w

Files in This Item:
File Description SizeFormat 
Sun et al 2019 REVISED FINAL.pdfFulltext - Accepted Version2.05 MBAdobe PDFView/Open



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.