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dc.contributor.authorTocher, Douglas R-
dc.contributor.authorBell, J Gordon-
dc.contributor.authorDick, James R-
dc.contributor.authorCrampton, Viv-
dc.description.abstractFatty acyl desaturase activities, involved in the conversion of the C18 EFA, 18:2n-6 and 18:3n-3, to the highly unsaturated fatty acids (HUFA) 20:4n-6, 20:5n-3 and 22:6n-3, are known to be under nutritional regulation. Specifically, the activity of the desaturation/elongation pathway is depressed when animals, including fish, are fed fish oils rich in n-3HUFA compared to animals fed vegetable oils rich in C18 EFA. The primary aims of the present study were a) to establish the relative importance of product inhibition (n-3HUFA) versus increased substrate concentration (C18 EFA) and, b) to determine whether 18:2n-6 and 18:3n-3 differ in their effects, on the hepatic fatty acyl desaturation/elongation pathway in Atlantic salmon (Salmo salar). Smolts were fed ten experimental diets containing blends of two vegetable oils, linseed (LO) and rapeseed oil (RO), and fish oil (FO) in a triangular mixture design for 50 weeks. Fish were sampled after 32 and 50 weeks, lipid and fatty acid composition of liver determined, fatty acyl desaturation/elongation activity estimated in hepatocytes using [1-14C]18:3n-3 as substrate, and the data subjected to regression analyses. Dietary 18:2n-6 was positively correlated, and n-3HUFA negatively correlated, with lipid content of liver. Dietary 20:5n-3 and 22:6n-3 were positively correlated with liver fatty acids with a slope greater than unity suggesting relative retention and deposition of these HUFA. In contrast, dietary 18:2n-6 and 18:3n-3 were positively correlated with liver fatty acids with a slope of less than unity suggesting metabolism via β-oxidation and/or desaturation/elongation. Consistent with this, fatty acyl desaturation/elongation in hepatocytes was significantly increased by feeding diets containing vegetable oils. Dietary 20:5n-3 and 22:6n-3 levels were negatively correlated with hepatocyte fatty acyl desaturation. At 32 weeks, 18:2n-6 but not 18:3n-3, was positively correlated with hepatocyte fatty acyl desaturation activity whereas the reverse was true at 50 weeks. The data indicate that both feedback inhibition through increased n-3HUFA and decreased C18 fatty acyl substrate concentration are probably important in determining hepatocyte fatty acyl desaturation activities, and that 18:2n-6 and 18:3n-3 may differ in their effects on this pathway.en_UK
dc.publisherSpringer / American Oil Chemists' Society (AOCS)-
dc.relationTocher DR, Bell JG, Dick JR & Crampton V (2003) Effects of dietary vegetable oil on atlantic salmon hepatocyte fatty acid desaturation and liver fatty acid compositions, Lipids, 38 (7), pp. 723-732.-
dc.rightsPublished in Lipids by Springer / American Oil Chemists' Society (AOCS).; The final publication is available at
dc.subjectAtlantic salmonen_UK
dc.subjectSalmo salaren_UK
dc.subjectFish oilen_UK
dc.subjectVegetable oilen_UK
dc.subjectLinseed oilen_UK
dc.subjectRapeseed oilen_UK
dc.subjectMixture designen_UK
dc.subjectLipid metabolismen_UK
dc.subjectPolyunsaturated fatty aciden_UK
dc.subject.lcshAtlantic salmon-
dc.subject.lcshLipids in nutrition-
dc.subject.lcshFishes Feeding and feeds-
dc.titleEffects of dietary vegetable oil on atlantic salmon hepatocyte fatty acid desaturation and liver fatty acid compositionsen_UK
dc.typeJournal Articleen_UK
dc.type.statusPost-print (author final draft post-refereeing)-
dc.contributor.affiliationEWOS Innovation-
Appears in Collections:Aquaculture Journal Articles

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