Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorGhioni, Cristinaen_UK
dc.description.abstractMarine fish are known to have an absolute dietary requirement for C20 and C22 highly unsaturated fatty acids. Previous studies using cultured cell lines indicated that underlying this requirement in marine fish was either a deficiency in fatty acyl Δ5 desaturase or C18-20 elongase activity. Recently, Ghioni et al. (Biochim. Biophys. Acta, 1437, 170-181, 1999) presented evidence that in turbot cells there was low activity of C18-20 elongase whereas Δ5 desaturase had high activity. In the present study, the fatty acid desaturase/elongase pathway was investigated in a cell line (SAF-1) from another carnivorous marine fish, sea bream. The metabolic conversions of a range of radiolabelled polyunsaturated fatty acids that comprised the direct substrates for Δ6 desaturase ([1-14C]18:2n-6 and [1-14C]18:3n-3), C18-20 elongase ([U-14C]18:4n-3), Δ5 desaturase ([1-14C]20:3n-6 and [U-14C]20:4n-3) and C20-22 elongase ([1-14C]20:4n-6 and [1-14C]20:5n-3) were utilized. The results showed that fatty acyl Δ6 desaturase in SAF-1 cells was highly active and there was substantial C18-20 elongase and C20-22 elongase activities. A deficiency in the desaturation/elongation pathway was clearly identified at the level of the fatty acyl Δ5 desaturase which was very low, particularly with 20:4n-3 as substrate. In comparison, the apparent activities of Δ6 desaturase, C18-20 elongase and C20-22 elongase were approximately 94-fold, 27-fold and 16-fold greater than that for Δ5 desaturase towards their respective n-3 polyunsaturated fatty acid substrates. The evidence obtained in the SAF-1 cell line is consistent with the dietary requirement for C20 and C22 highly unsaturated fatty acids in the marine fish, the sea bream, being primarily due to a deficiency in fatty acid Δ5 desaturase activity.en_UK
dc.publisherSpringer / American Oil Chemists' Society (AOCS)en_UK
dc.relationTocher DR & Ghioni C (1999) Fatty acid metabolism in marine fish: Low activity of fatty acyl Δ5 desaturation in gilthead sea bream ( Sparus aurata ) cells. Lipids, 34 (5), pp. 433-440.
dc.rightsPublished in Lipids by Springer / American Oil Chemists' Society (AOCS).; The original publication is available at www.springerlink.comen_UK
dc.subjectCell cultureen_UK
dc.subjectGilthead seabreamen_UK
dc.subjectSAF-1 cell lineen_UK
dc.subjectFatty acid metabolismen_UK
dc.subjectPolyunsaturated fatty acidsen_UK
dc.subjectDelta-5 desaturaseen_UK
dc.subjectFish oilsen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.titleFatty acid metabolism in marine fish: Low activity of fatty acyl Δ5 desaturation in gilthead sea bream ( Sparus aurata ) cellsen_UK
dc.typeJournal Articleen_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorGhioni, Cristina|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameSAF D5 Paper Final.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
SAF D5 Paper Final.pdfFulltext - Accepted Version197.28 kBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.