Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29235
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dc.contributor.authorJääskeläinen, Anne Jen_UK
dc.contributor.authorSironen, Tarjaen_UK
dc.contributor.authorDiagne, Cheikh Tidianeen_UK
dc.contributor.authorDiagne, Moussa Moïseen_UK
dc.contributor.authorFaye, Martinen_UK
dc.contributor.authorFaye, Oumaren_UK
dc.contributor.authorFaye, Ousmaneen_UK
dc.contributor.authorHewson, Rogeren_UK
dc.contributor.authorMölsä, Markosen_UK
dc.contributor.authorWeidmann, Manfred Wen_UK
dc.contributor.authorWatson, Roberten_UK
dc.contributor.authorSall, Amadou Alphaen_UK
dc.contributor.authorVapalahti, Ollien_UK
dc.date.accessioned2019-04-06T00:00:38Z-
dc.date.available2019-04-06T00:00:38Z-
dc.date.issued2019-05en_UK
dc.identifier.urihttp://hdl.handle.net/1893/29235-
dc.description.abstractBackground During the five decades since their discovery, filoviruses of four species have caused human hemorrhagic fever outbreaks: Marburg (MARV) marburgvirus, and Zaire (EBOV), Sudan (SUDV) and Bundybugyo (BDBV) ebolaviruses. The largest, devastating EBOV epidemic in West Africa in 2014-16, has been followed by outbreaks of MARV in Uganda, 2017, and EBOV in Democratic Republic of Congo, 2018, emphasizing the need to develop preparedness to diagnose all filoviruses. Objectives The aim of this study was to optimize a new filovirus RT-qPCR to detect all filoviruses, define its limits of detection (LOD) and perform a field evaluation with outbreak samples. Study design A pan-filovirus RT-qPCR targeting the L gene was developed and evaluated within the EbolaMoDRAD (Ebola virus: modern approaches for developing bedside rapid diagnostics) project. Specificity and sensitivity were determined and the effect of inactivation and PCR reagents (liquid and lyophilized format) were tested. Results The LODs for the lyophilized pan-filovirus L-RT-qPCR assay were 9.4 copies per PCR reaction for EBOV, 9.9 for MARV, 1151 for SUDV, 65 for BDBV and 289 for Taï Forest virus. The test was set at the Pasteur Institute, Dakar, Senegal, and 83 Ebola patient samples, with viral load ranging from 5 to 5 million copies of EBOV per reaction, were screened. The results for the patient samples were in 100% concordance with the reference EBOV-specific assay. Discussion Overall, the assay showed good sensitivity and specificity, covered all filoviruses known to be human pathogens, performed well both in lyophilized and liquid-phase formats and with EBOV outbreak clinical samples.en_UK
dc.language.isoenen_UK
dc.publisherElsevier BVen_UK
dc.relationJääskeläinen AJ, Sironen T, Diagne CT, Diagne MM, Faye M, Faye O, Faye O, Hewson R, Mölsä M, Weidmann MW, Watson R, Sall AA & Vapalahti O (2019) Development, validation and clinical evaluation of a broad-range pan-filovirus RT-qPCR. Journal of Clinical Virology, 114, pp. 26-31. https://doi.org/10.1016/j.jcv.2019.03.010en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Jääskeläinen AJ, Sironen T, Diagne CT, Diagne MM, Faye M, Faye O, Faye O, Hewson R, Mölsä M, Weidmann MW, Watson R, Sall AA & Vapalahti O (2019) Development, validation and clinical evaluation of a broad-range pan-filovirus RT-qPCR. Journal of Clinical Virology, 114, pp. 26-31. DOI: https://doi.org/10.1016/j.jcv.2019.03.010 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectEbolaen_UK
dc.subjectMarburgen_UK
dc.subjectSudanen_UK
dc.subjectBundibugyoen_UK
dc.subjectPan-Filoen_UK
dc.titleDevelopment, validation and clinical evaluation of a broad-range pan-filovirus RT-qPCRen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2020-03-20en_UK
dc.rights.embargoreason[Pan-filo_manuscript_revision_submitted.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.jcv.2019.03.010en_UK
dc.identifier.pmid30904708en_UK
dc.citation.jtitleJournal of Clinical Virologyen_UK
dc.citation.issn1386-6532en_UK
dc.citation.volume114en_UK
dc.citation.spage26en_UK
dc.citation.epage31en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderHelsinki University Hospitalen_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.citation.date19/03/2019en_UK
dc.contributor.affiliationUniversity of Helsinkien_UK
dc.contributor.affiliationUniversity of Helsinkien_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationPublic Health Englanden_UK
dc.contributor.affiliationNational Institute for Health and Welfare, Finlanden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationPublic Health Englanden_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationUniversity of Helsinkien_UK
dc.identifier.scopusid2-s2.0-85063109282en_UK
dc.identifier.wtid1264413en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2019-03-15en_UK
dc.date.filedepositdate2019-04-05en_UK
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