Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2920
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dc.contributor.authorMourente, Gabrielen_UK
dc.contributor.authorDiaz-Salvago, Estheren_UK
dc.contributor.authorBell, J Gordonen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2013-10-08T02:12:10Z-
dc.date.available2013-10-08T02:12:10Z-
dc.date.issued2002-11en_UK
dc.identifier.urihttp://hdl.handle.net/1893/2920-
dc.description.abstractPreviously, we had shown that altering the highly unsaturated fatty acid (HUFA)/vitamin E ratios in gilthead sea bream livers significantly affected their peroxidation status, with fish fed a diet rich in HUFA and low in vitamin E showing significantly higher values of lipid peroxidation products, without, however, significant effects on liver antioxidant defence enzyme activities. The aim of the present trial was to further characterise the biochemical indicators of peroxidative stress in juvenile gilthead sea bream. A high pro-oxidative stress was induced by feeding diets containing around 7% of the dry weight as n 3 HUFA. The potential peroxidative stress was increased by oxidising the oil, increasing the peroxide value of the oil some 10-fold. These oils were fed without or with supplemental vitamin E (a-tocopheryl acetate at 200 mg kg 1 dry diet) giving four diets in total. Fish were sampled after 30 and 60 days of feeding the experimental diets. None of the diets had any serious deleterious effects on growth and mortality of the fish during the trial. Similarly, there were few significant effects due to dietary oxidised oil or supplementary vitamin E on liver lipid and fatty acid profiles and, in particular, the proportions of HUFA were not decreased by dietary oxidised oil. The vitamin E content of the liver reflected the vitamin E content of the diets but was also affected by dietary oxidised oil being reduced by oxidised oil in fish fed diets without supplemental vitamin E but, unexpectedly, increased by oxidised oil in fish fed diets supplemented with vitamin E. Liver thiobarbituric acid reactive substances (TBARS) levels were significantly lower in fish fed diets supplemented with vitamin E whereas dietary oxidised oil had no major effect on lipid peroxidation products. Catalase (CAT) and superoxide dismutase (SOD) activities were both increased in fish fed dietary oxidised oil and reduced by supplementary vitamin E after 30 days feeding. In contrast, glutathione peroxidase (GPX) was less affected by the diets, and the activities of glutathione-Stransferase (GST) and glutathione reductase (GR) were only reduced by dietary vitamin E after 60 days of feeding. However, all the enzyme activities were significantly affected by the duration of feeding, but the number of interactions between the three factors (time, oil and vitamin E) showed that the relationships were complicated. In conclusion, the present study showed that feeding diets containing oxidised oil significantly affected the activities of liver antioxidant defence enzymes and that dietary vitamin E partially abrogated these effects. Growth and survival of the fish were relatively unaffected suggesting that the responses in gilthead sea bream offered effective protection. However, the duration of feeding the diets of high pro-oxidative stress was observed to have a hitherto unknown effect, possibly the result of an adaptive process, but which requires further investigation.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMourente G, Diaz-Salvago E, Bell JG & Tocher DR (2002) Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream (Sparus aurata L.) fed dietary oxidised oil: attenuation by dietary vitamin E. Aquaculture, 214 (41000), pp. 343-361. http://www.sciencedirect.com/science/journal/00448486; https://doi.org/10.1016/S0044-8486%2802%2900064-9en_UK
dc.rightsPublished in Aquaculture by Elsevier. Aquaculture, Volume 214, Issues 1-4, November 2002, pp. 343 - 361.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Aquaculture. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquaculture, VOL 214, ISSUE 1-4, (November 2002). DOI 10.1016/S0044-8486(02)00064-9.en_UK
dc.subjectGilthead seabreamen_UK
dc.subjectSparus aurataen_UK
dc.subjectalpha-tocopherolen_UK
dc.subjectVitamin Een_UK
dc.subjectOxidised oilen_UK
dc.subjectPolyunsaturated fatty acidsen_UK
dc.subjectPeroxidationen_UK
dc.subjectAntioxidant defence enzymesen_UK
dc.subjectMDAen_UK
dc.subjectIsoprostanesen_UK
dc.subjectFishes Nutrition Requirementsen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectFishes Nutrition Requirmentsen_UK
dc.titleIncreased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream (Sparus aurata L.) fed dietary oxidised oil: attenuation by dietary vitamin Een_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/S0044-8486(02)00064-9en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume214en_UK
dc.citation.issue41000en_UK
dc.citation.spage343en_UK
dc.citation.epage361en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.identifier.urlhttp://www.sciencedirect.com/science/journal/00448486en_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.citation.date21/03/2002en_UK
dc.contributor.affiliationUniversity of Cadizen_UK
dc.contributor.affiliationCentro Andaluz Superior de Estudios Marinosen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000178793800023en_UK
dc.identifier.scopusid2-s2.0-0037111039en_UK
dc.identifier.wtid839141en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2002-03-21en_UK
dc.date.filedepositdate2011-04-14en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMourente, Gabriel|en_UK
local.rioxx.authorDiaz-Salvago, Esther|en_UK
local.rioxx.authorBell, J Gordon|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2011-04-14en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2011-04-14|en_UK
local.rioxx.filenameEUCadiz DT4 final.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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