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dc.contributor.authorHastings, Nicolaen_UK
dc.contributor.authorAgaba, Morris Ken_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorZheng, Xiaozhongen_UK
dc.contributor.authorDickson, Cathrynen_UK
dc.contributor.authorDick, James Ren_UK
dc.contributor.authorTeale, Alan Jen_UK
dc.description.abstractFish are the only major dietary source for humans of omega-3 highly unsaturated fatty acids (HUFA) and, with declining fisheries, farmed fish such as Atlantic salmon (Salmo salar) constitute an increasing proportion of the fish in the human diet. However, the current high use of fish oils, derived from wild capture marine fisheries, in aquaculture feeds is not sustainable in the longer term, and will constrain continuing growth of aquaculture activities. A greater understanding of how fish metabolise and biosynthesise HUFA may lead to effective use of more sustainable aquaculture diets. The study described here contributes to an effort to determine the molecular genetics of the HUFA biosynthetic pathway in salmon, with the overall aim being to determine mechanisms for optimising the use of vegetable oils in Atlantic salmon culture. In this paper we describe the cloning and functional characterisation of two genes from salmon involved in the biosynthesis of HUFA. A salmon desaturase cDNA, SalDes, was isolated that included an open reading frame (ORF) of 1362 bp specifying a protein of 454 amino acids. The protein sequence included all the characteristic features of microsomal fatty acid desaturases, including three histidine boxes, two transmembrane regions, and an N-terminal cytochrome b5 domain containing a haem-binding motif similar to that of other fatty acid desaturases. Functional expression in the yeast, Saccharomyces cerevisiae, showed SalDes is predominantly an omega-3 Δ5 desaturase, a key enzyme in the synthesis of eicosapentaenoic acid (20:5n-3) from α-linolenic acid (18:3n-3). The desaturase showed only low levels of Δ6 activity towards C18 polyunsaturated fatty acids. In addition, a fatty acid elongase cDNA, SalElo, was isolated that includes an ORF of 888 bp, specifying a protein of 295 amino acids. The protein sequence of SalElo includes characteristic features of microsomal fatty acid elongases, including a histidine box and a transmembrane region. Upon expression in yeast, SalElo showed broad substrate specificity for polyunsaturated fatty acids with a range of chain lengths, with the rank order being C18 > C20 > C22. Thus, all fatty acid elongase activities required for the biosynthesis of docosahexaenoic acid (22:6n-3) from 18:3n-3 are displayed by this one polypeptide product.en_UK
dc.relationHastings N, Agaba MK, Tocher DR, Zheng X, Dickson C, Dick JR & Teale AJ (2004) Molecular cloning and functional characterization of fatty acyl desaturase and elongase cDNAs involved in the production of eicosapentaenoic and docosahexanoic acids from alpha-linolenic acid in Atlantic salmon (Salmo salar). Marine Biotechnology, 6 (5), pp. 463-474.;
dc.rightsPublished in Marine Biotechnology by Springer.; The final publication is available at www.springerlink.comen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectSalmo salaren_UK
dc.subjectPolyunsaturated fatty acidsen_UK
dc.subjectDelta-5 Fatty acyl desaturaseen_UK
dc.subjectElovl5 elongaseen_UK
dc.subjectcDNA cloningen_UK
dc.subjectFunctional characterisationen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectOmega-3 fatty acidsen_UK
dc.subjectUnsaturated fatty acids in human nutritionen_UK
dc.titleMolecular cloning and functional characterization of fatty acyl desaturase and elongase cDNAs involved in the production of eicosapentaenoic and docosahexanoic acids from alpha-linolenic acid in Atlantic salmon (Salmo salar)en_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleMarine Biotechnologyen_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorHastings, Nicola|en_UK
local.rioxx.authorAgaba, Morris K|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorZheng, Xiaozhong|en_UK
local.rioxx.authorDickson, Cathryn|en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.authorTeale, Alan J|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameSalmongenes final.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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