Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29178
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dc.contributor.authorSirimanapong, Wannaen_UK
dc.contributor.authorThompson, Kimen_UK
dc.contributor.authorKledmanee, Kanen_UK
dc.contributor.authorThaijongrak, Prawpornen_UK
dc.contributor.authorCollet, Bertranden_UK
dc.contributor.authorOoi, Ei Linen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.date.accessioned2019-04-02T00:05:19Z-
dc.date.available2019-04-02T00:05:19Z-
dc.date.issued2014-10-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/29178-
dc.description.abstractAquaculture production of Pangasianodon hypophthalmus is growing rapidly in South East Asia, especially in Vietnam. As it is a relatively new aquaculture species there are few reports evaluating its immune response to pathogens. Thus, functional assays for P.hypophthalmus were optimised to evaluate both innate and adaptive immune responses, and were then used to examine immune response following stimulation with live and heat-killed Aeromonas hydrophila. These were used as models of infection and vaccination, respectively. Four treatment groups were used, including a control group, a group injected intraperitonally (IP) with adjuvant only, a group injected with heat-killed A.hydrophila (1×109cfuml-1 mixed with adjuvant), and a group injected with a subclinical dose of live A.hydrophila. Samples were collected at 0, 1, 3, 7, 14 and 21 days post-injection (d.p.i.) to assess their immune response. The results indicated that challenge with live or dead bacteria stimulated the immune response in P.hypophthalmus significantly above the levels observed in control groups with respect to specific antibody titre, plasma lysozyme and peroxidase activity, and phagocytosis by head kidney macrophages at 7 or/and 14d.p.i. At 21d.p.i., total and specific antibody (IgM) levels and plasma lysozyme activity in fish injected with either live or dead A.hydrophila were significantly different to the control groups. Differential immune responses were observed between fish injected with either live or dead bacteria, with live A.hydrophila significantly stimulating an increase in WBC counts and plasma peroxidase activity at 3d.p.i., with the greatest increase in WBC counts noted at 21d.p.i. and in phagocytosis at 14d.p.i. By 21d.p.i. only the macrophages from fish injected with dead A.hydrophila showed significantly stimulation in their respiratory burst activity. This study provides basic information on the immune response in pangasius catfish that can be useful in the health control of this species.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationSirimanapong W, Thompson K, Kledmanee K, Thaijongrak P, Collet B, Ooi EL & Adams A (2014) Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccination. Fish and Shellfish Immunology, 40 (2), pp. 374-383. https://doi.org/10.1016/j.fsi.2014.07.021en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectPangasianodon hypophthalmusen_UK
dc.subjectAeromonas hydrophilaen_UK
dc.subjectLive and killed bacteriaen_UK
dc.subjectInnate and adaptive immunityen_UK
dc.titleOptimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccinationen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.identifier.doi10.1016/j.fsi.2014.07.021en_UK
dc.identifier.pmid25064539en_UK
dc.citation.jtitleFish and Shellfish Immunologyen_UK
dc.citation.issn1095-9947en_UK
dc.citation.issn1050-4648en_UK
dc.citation.volume40en_UK
dc.citation.issue2en_UK
dc.citation.spage374en_UK
dc.citation.epage383en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailalexandra.adams@stir.ac.uken_UK
dc.citation.date24/07/2014en_UK
dc.contributor.affiliationMahidol Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationMahidol Universityen_UK
dc.contributor.affiliationMahidol Universityen_UK
dc.contributor.affiliationScottish Government - Enterprise, Environment & Digital - Marine Scotlanden_UK
dc.contributor.affiliationNovus Internationalen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000343381100004en_UK
dc.identifier.scopusid2-s2.0-84905915104en_UK
dc.identifier.wtid1078515en_UK
dc.date.accepted2014-07-10en_UK
dcterms.dateAccepted2014-07-10en_UK
dc.date.filedepositdate2018-12-20en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorSirimanapong, Wanna|en_UK
local.rioxx.authorThompson, Kim|en_UK
local.rioxx.authorKledmanee, Kan|en_UK
local.rioxx.authorThaijongrak, Prawporn|en_UK
local.rioxx.authorCollet, Bertrand|en_UK
local.rioxx.authorOoi, Ei Lin|en_UK
local.rioxx.authorAdams, Alexandra|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2264-06-25en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameA6_Optimisation and standardisation.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1095-9947en_UK
Appears in Collections:Aquaculture Journal Articles

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