Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2911
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dc.contributor.authorGhioni, Cristinaen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorBell, Michaelen_UK
dc.contributor.authorDick, James Ren_UK
dc.contributor.authorSargent, John Ren_UK
dc.date.accessioned2013-05-02T00:28:04Z-
dc.date.available2013-05-02T00:28:04Z-
dc.date.issued1999-02-25en_UK
dc.identifier.urihttp://hdl.handle.net/1893/2911-
dc.description.abstractThe TF cell line, derived from a top predatory, carnivorous marine teleost, the turbot (Scophthalmus maximus), is known to have a limited conversion of C18 to C20 polyunsaturated fatty acids (PUFA). To illuminate the underlying processes, we studied the conversions of stearidonic acid, 18:4(n-3), and its elongation product, 20:4(n-3), in TF cells and also in a cell line, AS, derived from Atlantic salmon (Salmo salar), by adding unlabelled (25 uM), U-14C (1 uM) or deuterated (d5; 25 uM) fatty acids. Stearidonic acid, 18:4(n-3), was metabolised to 20:5(n-3) in both cells lines, but more so in AS than in TF cells. Delta-5 desaturation was more active in TF cells than in AS cells, whereas C18 to C20 elongation was much reduced in TF as compared to AS cells. Only small amounts of docosahexaenoic acid (22:6(n-3)) were produced by both cell lines, although there was significant production of 22:5(n-3) in both cultures, especially when 20:4(n-3) was supplemented. We conclude that limited elongation of C18 to C20 fatty acids rather than limited fatty acyl Delta-5 desaturation accounts for the limited rate of conversion of 18:3(n-3) to 20:5(n-3) in the turbot cell line, as compared to the Atlantic salmon cell line. The results can account for the known differences in conversions of C18 to C20 PUFA by the turbot and the Atlantic salmon in vivo.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationGhioni C, Tocher DR, Bell M, Dick JR & Sargent JR (1999) Low C18 to C20 fatty acid elongase activity and limited conversion of stearidonic acid, 18:4(n-3), to eicosapentaenoic acid, 20:5(n-3), in a cell line from the turbot, Scophthalmus maximus. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1437 (2), pp. 170-181. https://doi.org/10.1016/S1388-1981%2899%2900010-4en_UK
dc.rightsPublished in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids by Elsevier. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1437, Issue 2, February 1999, pp. 170 - 181; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, VOL 1437, ISSUE 2, February 1999. DOI 10.1016/S1388-1981(99)00010-4en_UK
dc.subjectFishen_UK
dc.subjectCell cultureen_UK
dc.subjectFatty acidsen_UK
dc.subjectPolyunsaturated fatty aciden_UK
dc.subjectbiosynthesisen_UK
dc.subjectDesaturationen_UK
dc.subjectElongationen_UK
dc.subjectMetabolismen_UK
dc.subjectAS cell lineen_UK
dc.subjectTF cell lineen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectTurboten_UK
dc.subjectLipoproteins Fishen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectAtlantic salmonen_UK
dc.titleLow C18 to C20 fatty acid elongase activity and limited conversion of stearidonic acid, 18:4(n-3), to eicosapentaenoic acid, 20:5(n-3), in a cell line from the turbot, Scophthalmus maximusen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/S1388-1981(99)00010-4en_UK
dc.citation.jtitleBiochimica et Biophysica Acta Molecular and Cell Biology of Lipidsen_UK
dc.citation.issn1388-1981en_UK
dc.citation.volume1437en_UK
dc.citation.issue2en_UK
dc.citation.spage170en_UK
dc.citation.epage181en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.identifier.scopusid2-s2.0-0033601947en_UK
dc.identifier.wtid838950en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted1999-02-25en_UK
dc.date.filedepositdate2011-04-14en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorGhioni, Cristina|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorBell, Michael|en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.authorSargent, John R|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2011-04-14en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2011-04-14|en_UK
local.rioxx.filenameTFAS-Final.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1388-1981en_UK
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