Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2908
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dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorDick, James Ren_UK
dc.date.accessioned2013-06-08T23:34:02Z-
dc.date.available2013-06-08T23:34:02Z-
dc.date.issued2001-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/2908-
dc.description.abstractThe desaturation of [1-14C]18:3n-3 to docosahexaenoic acid (DHA; 22:6n-3) is enhanced in an essential fatty acid deficient cell line (EPC-EFAD) in comparison with the parent cell line (EPC) from carp. In the present study, the effects of DHA on lipid and fatty acid compositions, and the metabolism of [1-14C]18:3n-3 were investigated in EPC-EFAD cells in comparison with EPC cells. DHA supplementation had only relatively minor effects on lipid content and lipid class compositions in both EPC and EPC-EFAD cells, but significantly increased the amount of DHA, 22:5n-3, eicosapentaenoic acid (EPA; 20:5n-3), total n-3 polyunsaturated fatty acids (PUFA), total PUFA and saturated fatty acids in total lipid and total polar lipid in both cell lines. Retroconversion of supplemental DHA to EPA was significantly greater in EPC cells. Monounsaturated fatty acids, n-9 and n-6PUFA were all increased in total lipid and total polar lipid in both cell lines by DHA supplementation. The incorporation of [1-14C]18:3n-3 was greater into EPC-EFAD compared to EPC cells but DHA had no effect on the incorporation of [1-14C]18:3n-3 in either cell line. In contrast, the conversion of [1-14C]18:3n-3 to tetraenes, pentaenes and total desaturation products was similar in the two cell lines and was significantly reduced by DHA supplementation in both cell lines. However, the production of DHA from [1-14C]18:3n-3 was significantly greater in EPC-EFAD cells compared to EPC cells and, whereas DHA supplementation had no effect on the production of DHA from [1-14C]18:3n-3 in EPC cells, DHA supplementation significantly reduced the production of DHA from [1-14C]18:3n-3 in EPC-EFAD cells. Greater production of DHA in EPC-EFAD cells could be a direct result of significantly lower levels of end-product DHA in these cells’ lipids compared to EPC cells. Consistent with this, the suppression of DHA production upon DHA supplementation was associated with increased cellular and membrane DHA concentrations in EPC-EFAD cells. However, an increase in cellular DHA content to similar levels failed to suppress DHA production in DHA-supplemented EPC cells. A possible explanation is that greatly increased levels of EPA, derived from retroconversion of the added DHA, acts to offset the suppression of the pathway by DHA by stimulating conversion of EPA to DHA in DHA-supplemented EPC cells.en_UK
dc.language.isoenen_UK
dc.publisherElsevier (Harcourt Publishers Ltd.) / International Society for the Study of Fatty Acids and Lipidsen_UK
dc.relationTocher DR & Dick JR (2001) Effects of essential fatty acid deficiency and supplementation with docosahexaenoic acid (DHA; 22:6n-3) on cellular fatty acid compositions and fatty acyl desaturation in a cell culture model. Prostaglandins, Leukotrienes and Essential Fatty Acids, 64 (1), pp. 11-22. http://www.sciencedirect.com/science/journal/09523278; https://doi.org/10.1054/plef.2000.0233en_UK
dc.rightsPublished in Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) by Elsevier (Harcourt Publishers Ltd.) / International Society for the Study of Fatty Acids and Lipids.; Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA), Volume 64, Issue 1, January 2001, pp. 11 - 22.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA). Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA), VOL 64, ISSUE 1, (January 2001). DOI 10.1054/plef.2000.0233.en_UK
dc.subjectFishen_UK
dc.subjectCell cultureen_UK
dc.subjectEPCen_UK
dc.subjectEssential fatty acid deficiencyen_UK
dc.subjectCell modelen_UK
dc.subjectPolyunsaturated fatty acidsen_UK
dc.subjectMetabolismen_UK
dc.subjectDesaturationen_UK
dc.subjectElongationen_UK
dc.subjectDHAen_UK
dc.subjectNutritional disorders Fishesen_UK
dc.subjectCell culture Fishesen_UK
dc.titleEffects of essential fatty acid deficiency and supplementation with docosahexaenoic acid (DHA; 22:6n-3) on cellular fatty acid compositions and fatty acyl desaturation in a cell culture modelen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1054/plef.2000.0233en_UK
dc.citation.jtitleProstaglandins, Leukotrienes and Essential Fatty Acidsen_UK
dc.citation.issn0952-3278en_UK
dc.citation.volume64en_UK
dc.citation.issue1en_UK
dc.citation.spage11en_UK
dc.citation.epage22en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.identifier.urlhttp://www.sciencedirect.com/science/journal/09523278en_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000166781400003en_UK
dc.identifier.scopusid2-s2.0-0035126303en_UK
dc.identifier.wtid838916en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2001-01-31en_UK
dc.date.filedepositdate2011-04-14en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2011-04-14en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2011-04-14|en_UK
local.rioxx.filenameEFADDHA Final.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0952-3278en_UK
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