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dc.contributor.authorOxley, Anthonyen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorTorstensen, Bente Een_UK
dc.contributor.authorOlsen, Rolf Een_UK
dc.description.abstractA combined fatty acid metabolism assay was employed to determine fatty acid uptake and relative utilisation in enterocytes isolated from the pyloric caeca of rainbow trout. In addition, the effect of a diet high in long-chain monoenoic fatty alcohols present as wax esters in oil derived from Calanus finmarchicus, compared to a standard fish oil diet, on caecal enterocyte fatty acid metabolism was investigated. The diets were fed for 8 weeks before caecal enterocytes from each dietary group were isolated and incubated with [1-14C]fatty acids: 16:0, 18:1n-9, 18:2n-6, 18:3n-3, 20:1n-9, 20:4n-6, 20:5n-3, and 22:6n-3. Uptake was measured over 2 h with relative utilisation of different [1-14C]fatty acids calculated as a percentage of uptake. Differences in uptake were observed, with 18:1n-9 and 18:2n-6 showing the highest rates. Esterification into cellular lipids was highest with 16:0 and C18 fatty acids, accounting for over one-third of total uptake, through predominant incorporation in triacylglycerol (TAG). The overall utilisation of fatty acids in phospholipid synthesis was low, but highest with 16:0, the most prevalent fatty acid recovered in intracellular phosphatidylcholine (PC) and phosphatidylinositol (PI), although exported PC exhibited higher proportions of C20/C22 polyunsaturated fatty acids (PUFA). Other than 16:0, incorporation into PC and PI was highest with C20/C22 PUFA and 20:4n-6 respectively. Recovery of labelled 18:1n-9 in exported TAG was 3-fold greater than any other fatty acid which could be due to multiple esterification on the glycerol ‘backbone’ and/or increased export. Approximately 20-40% of fatty acids taken up were β-oxidised, and was highest with 20:4n-6. Oxidation of 20:5n-3 and 22:6n-3 was also surprisingly high, although 22:6n-3 oxidation was mainly attributed to retroconversion to 20:5n-3. Metabolic modification of fatty acids by elongation-desaturation was generally low aten_UK
dc.relationOxley A, Tocher DR, Torstensen BE & Olsen RE (2005) Fatty acid utilisation and metabolism in caecal enterocytes of rainbow trout (Oncorhynchus mykiss) fed dietary fish or copepod oil. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1737 (2-3), pp. 119-129.
dc.rightsPublished in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids by Elsevier. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1737, Issues 2-3, December 2005, pp. 119 - 129.; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, VOL 1737, ISSUE 2-3, (December 2005). DOI 10.1016/j.bbalip.2005.09.008.en_UK
dc.subjectRainbow trouten_UK
dc.subjectOncorhynchus mykissen_UK
dc.subjectFish oilen_UK
dc.subjectCopepod oilen_UK
dc.subjectLipid and fatty acid metabolismen_UK
dc.subjectWax estersen_UK
dc.subjectFatty alcoholsen_UK
dc.subjectRainbow trouten_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectFishes Nutritionen_UK
dc.titleFatty acid utilisation and metabolism in caecal enterocytes of rainbow trout (Oncorhynchus mykiss) fed dietary fish or copepod oilen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleBiochimica et Biophysica Acta Molecular and Cell Biology of Lipidsen_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationMatre Aquaculture Research Stationen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNational Institute of Nutrition and Seafood Research (NIFES)en_UK
dc.contributor.affiliationMatre Aquaculture Research Stationen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorOxley, Anthony|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorTorstensen, Bente E|en_UK
local.rioxx.authorOlsen, Rolf E|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameOxleyet al final.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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