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dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorFonseca-Madrigal, Jorgeen_UK
dc.contributor.authorBell, J Gordonen_UK
dc.contributor.authorDick, James Ren_UK
dc.contributor.authorHenderson, R Jamesen_UK
dc.contributor.authorSargent, John Ren_UK
dc.description.abstractWe hypothesized that replacing fish oil with 18:3n-3-rich linseed oil may enable salmon to maintain the levels of tissue n-3HUFA levels through a combination of increased desaturation activity and increased substrate fatty acid provision. To this end we investigated desaturation/elongation of [1-14C18:3n-3 in hepatocytes and intestinal enterocytes, and determined the extent to which 18:3n-3 was oxidized and desaturated by measuring both simultaneously in a combined assay. Salmon smolts were stocked randomly into five seawater pens and fed for 40 weeks on diets in which the fish oil was replaced in a graded manner by linseed oil. At the end of the trial, fatty acyl desaturation/elongation and oxidation activities were determined in isolated hepatocytes and intestinal enterocytes using [1-14C]18:3n-3 as substrate, and samples of liver and intestinal tissue were collected for analysis of lipid and fatty acid composition. The results showed that, despite increased desaturation of [1-14C]18:3n-3 in hepatocytes, provision of dietary 18:3n-3 did not prevent the decrease in tissue n-3HUFA in fish fed linseed oil. Intestinal enterocytes were a site of significant fatty acid desaturation but, in contrast to hepatocytes, the activity was not increased by feeding linseed oil and was generally lower in fish fed linseed oil compared to fish fed only fish oil. In contrast, oxidation of [1-14C]18:3n-3 in enterocytes was generally increased in fish fed linseed oil compared to fish fed the diet containing only fish oil. However, oxidation of [1-14C]18:3n-3 in hepatocytes was 4- to 8-fold lower than in enterocytes and was not affected by diet. Furthermore, oxidation of [1-14C]18:3n-3 in enterocytes exceeded desaturation irrespective of dietary treatment, whereas similar amounts of [1-14C]18:3n-3 were desaturated and oxidized in hepatocytes from fish fed only fish oil and desaturation exceeded oxidation by 3-fold in fish fed the diet containing 100% linseed oil. The molecular mechanisms underpinning these results were discussed.en_UK
dc.relationTocher DR, Fonseca-Madrigal J, Bell JG, Dick JR, Henderson RJ & Sargent JR (2002) Effects of diets containing linseed oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes in Atlantic salmon (Salmo salar). Fish Physiology and Biochemistry, 26 (2), pp. 157-170.;
dc.rightsPublished in Fish Physiology and Biochemistry by Springer.; The final publication is available at www.springerlink.comen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectSalmo salaren_UK
dc.subjectFish oilen_UK
dc.subjectVegetable oilen_UK
dc.subjectLinseed oilen_UK
dc.subjectPolyunsaturated fatty acidsen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectFishes Nutrition Requirementsen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectLinseed oilen_UK
dc.titleEffects of diets containing linseed oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes in Atlantic salmon (Salmo salar)en_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleFish Physiology and Biochemistryen_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationMichoacan University of San Nicolas de Hildalgo (UMSNH)en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorFonseca-Madrigal, Jorge|en_UK
local.rioxx.authorBell, J Gordon|en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.authorHenderson, R James|en_UK
local.rioxx.authorSargent, John R|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameRAFOA I Final.pdfen_UK
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