Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/28779
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Molecular mechanisms underpinning transgenerational plasticity in the green sea urchin Psammechinus miliaris
Author(s): Clark, Melody S
Suckling, Colleen C
Cavallo, Allesandro
Mackenzie, Clara L
Thorne, Michael A S
Davies, Andrew J
Peck, Lloyd S
Issue Date: 30-Jan-2019
Citation: Clark M, Suckling C, Cavallo A, Mackenzie C, Thorne M, Davies A & Peck L (2019) Molecular mechanisms underpinning transgenerational plasticity in the green sea urchin Psammechinus miliaris. Scientific Reports, 9 (1), Art. No.: 952. https://doi.org/10.1038/s41598-018-37255-6.
Abstract: The pre-conditioning of adult marine invertebrates to altered conditions, such as low pH, can significantly impact offspring outcomes, a process which is often referred to as transgenerational plasticity (TGP). This study describes for the first time, the gene expression profiles associated with TGP in the green sea urchin Psammechinus miliaris and evaluates the transcriptional contribution to larval resilience. RNA-Seq was used to determine how the expression profiles of larvae spawned into low pH from pre-acclimated adults differed to those of larvae produced from adults cultured under ambient pH. The main findings demonstrated that adult conditioning to low pH critically pre-loads the embryonic transcriptional pool with antioxidants to prepare the larvae for the “new” conditions. In addition, the classic cellular stress response, measured via the production of heat shock proteins (the heat shock response (HSR)), was separately evaluated. None of the early stage larvae either spawned in low pH (produced from both ambient and pre-acclimated adults) or subjected to a separate heat shock experiment were able to activate the full HSR as measured in adults, but the capacity to mount an HSR increased as development proceeded. This compromised ability clearly contributes to the vulnerability of early stage larvae to acute environmental challenge
DOI Link: 10.1038/s41598-018-37255-6
Rights: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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