Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/28728
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dc.contributor.authorPoppi, David Aen_UK
dc.contributor.authorMoore, Stephen Sen_UK
dc.contributor.authorWade, Nicholas Men_UK
dc.contributor.authorGlencross, Brett Den_UK
dc.date.accessioned2019-02-09T01:00:12Z-
dc.date.available2019-02-09T01:00:12Z-
dc.date.issued2019-02-25en_UK
dc.identifier.urihttp://hdl.handle.net/1893/28728-
dc.description.abstractThe effects of dietary methionine (Met) supply on the postprandial pattern of plasma free amino acids and the differential expression of several genes associated with a number of sulfur amino acid and protein turnover pathways in the liver of juvenile barramundi (Lates calcarifer) was investigated. At the conclusion of a 49-day growth trial assessing the requirement for dietary Met, three treatments were selected (with deficient (DEF; 8.6 g kg−1), adequate (ADQ; 14.9 g kg−1) and excessive (EXC; 21.4 g kg−1)) levels of dietary Met, based on their respective growth responses. A peak occurred in plasma free Met at 2 h post-feeding in fish fed the DEF and ADQ diets and at 4 h post-feeding in fish in the EXC treatment. Liver samples collected at these timepoints, as well as those taken as a pre-feeding control, were analyzed for expression of genes involved in Met turnover (CGL, MAT-1, MAT-2a) and taurine biosynthetic pathways (CSAD, ADO, CDO), target of rapamycin inhibition (Redd-1), the somatotropic axis (GHR-II, IGFI, IGF-II) and protein turnover pathways (MUL-1, ZFAND-5). Markers of sulfur amino acid turnover were more significantly affected by time after feeding than by dietary Met level, suggesting production of these enzymes may be primarily regulated by the consumption of feed or protein, rather than by the dietary composition. Further, metabolised Met appeared likely to have been directed through S-Adenosylmethionine (SAM) dependent pathways, rather than converted to Cys, which may have contributed to the observed growth response. Both genes influencing the conversion of Met to SAM appear to be active at this lifestage in barramundi. Previously described markers of proteolytic pathways appear to be conserved in this species and we have confirmed that ZFAND-5 is a reliable biomarker of this process in barramundi. A number of important genes were investigated for the first time in this species and shown to be nutritionally regulated.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationPoppi DA, Moore SS, Wade NM & Glencross BD (2019) Postprandial plasma free amino acid profile and hepatic gene expression in juvenile barramundi (Lates calcarifer) is more responsive to feed consumption than to dietary methionine inclusion. Aquaculture, 501, pp. 345-358. https://doi.org/10.1016/j.aquaculture.2018.11.044.en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Poppi DA, Moore SS, Wade NM & Glencross BD (2019) Postprandial plasma free amino acid profile and hepatic gene expression in juvenile barramundi (Lates calcarifer) is more responsive to feed consumption than to dietary methionine inclusion. Aquaculture, 501, pp. 345-358. DOI: https://doi.org/10.1016/j.aquaculture.2018.11.044 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectBarramundien_UK
dc.subjectLates calcariferen_UK
dc.subjectMethionineen_UK
dc.subjectPostprandialen_UK
dc.subjectNutrigenomicsen_UK
dc.subjectProtein turnoveren_UK
dc.titlePostprandial plasma free amino acid profile and hepatic gene expression in juvenile barramundi (Lates calcarifer) is more responsive to feed consumption than to dietary methionine inclusionen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2019-11-19en_UK
dc.rights.embargoreason[Poppi et al 2019 - Postprandial Methionine-AAV.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.aquaculture.2018.11.044en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume501en_UK
dc.citation.spage345en_UK
dc.citation.epage358en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderCommonwealth Scientific Industrial Research Organisationen_UK
dc.author.emailb.d.glencross@stir.ac.uken_UK
dc.citation.date19/11/2018en_UK
dc.contributor.affiliationUniversity of Queenslanden_UK
dc.contributor.affiliationUniversity of Queenslanden_UK
dc.contributor.affiliationCSIRO Agriculture and Fooden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isi000453540800044en_UK
dc.identifier.scopusid2-s2.0-85057305817en_UK
dc.identifier.wtid1079202en_UK
dc.contributor.orcid0000-0003-1167-8530en_UK
dc.date.accepted2018-11-18en_UK
dc.date.firstcompliantdepositdate2019-02-08en_UK
dc.description.refREF Compliant by Deposit in Stirling's Repositoryen_UK
Appears in Collections:Aquaculture Journal Articles

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