Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/28658
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dc.contributor.authorChen, Cuiyingen_UK
dc.contributor.authorWang, Shuqien_UK
dc.contributor.authorZhang, Meien_UK
dc.contributor.authorChen, Baojiaen_UK
dc.contributor.authorYou, Cuihongen_UK
dc.contributor.authorXie, Dizhien_UK
dc.contributor.authorLiu, Yangen_UK
dc.contributor.authorMonroig, Óscaren_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorWaiho, Khoren_UK
dc.contributor.authorLi, Yuanyouen_UK
dc.date.accessioned2019-01-30T15:41:46Z-
dc.date.available2019-01-30T15:41:46Z-
dc.date.issued2019-05en_UK
dc.identifier.urihttp://hdl.handle.net/1893/28658-
dc.description.abstractRecently, microRNAs (miRNAs) have emerged as crucial regulators of lipid metabolism. However, the miRNA-mediated regulatory mechanism on long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA) biosynthesis in vertebrates remains largely unknown. Here, we address a potentially important role of miRNA-24 (miR-24) in the regulation of LC-PUFA biosynthesis in rabbitfish Siganus canaliculatus. miR-24 showed significantly higher abundance in liver of rabbitfish reared in brackish water than in seawater for fish fed vegetable oil diets and in S. canaliculatus hepatocyte line (SCHL) cells incubated with alpha-linolenic acid (ALA) than the control group. Similar expression patterns were also observed on the expression of sterol regulatory element-binding protein-1 (srebp1) and LC-PUFA biosynthesis related genes. While opposite results were observed on the expression of insulin-induced gene 1 (insig1), an endoplasmic reticulum membrane protein blocking Srebp1 proteolytic activation. Luciferase reporter assays revealed rabbitfish insig1 as a target of miR-24. Knockdown of miR-24 in SCHL cells resulted in increased Insig1 protein, and subsequently reduced mature Srebp1 protein and expression of genes required for LC-PUFA biosynthesis, and these effects could be attenuated after additional insig1 knockdown. Opposite results were observed with overexpression of miR-24. Moreover, increasing endogenous insig1 by knockdown of miR-24 inhibited Srebp1 processing and consequently suppressed LC-PUFA biosynthesis in rabbitfish hepatocytes. These results indicate a potentially critical role for miR-24 in regulating LC-PUFA biosynthesis through the Insig1/Srebp1 pathway by targeting insig1. This is the first report of miR-24 involved in LC-PUFA biosynthesis and thus may provide knowledge on the regulatory mechanisms of LC-PUFA biosynthesis in vertebrates.en_UK
dc.language.isoenen_UK
dc.publisherElsevier BVen_UK
dc.relationChen C, Wang S, Zhang M, Chen B, You C, Xie D, Liu Y, Monroig Ó, Tocher DR, Waiho K & Li Y (2019) miR-24 is involved in vertebrate LC-PUFA biosynthesis as demonstrated in marine teleost Siganus canaliculatus. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864 (5), pp. 619-628. https://doi.org/10.1016/j.bbalip.2019.01.010en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Chen C, Wang S, Zhang M, Chen B, You C, Xie D, Liu Y, Monroig Ó, Tocher DR, Waiho K & Li Y (2019) miR-24 is involved in vertebrate LC-PUFA biosynthesis as demonstrated in marine teleost Siganus canaliculatus. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864 (5), pp. 619-628. DOI: https://doi.org/10.1016/j.bbalip.2019.01.010 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectmiR-24en_UK
dc.subjectInsig1en_UK
dc.subjectSrebp1en_UK
dc.subjectLC-PUFA biosynthesisen_UK
dc.subjectSiganus canaliculatusen_UK
dc.titlemiR-24 is involved in vertebrate LC-PUFA biosynthesis as demonstrated in marine teleost Siganus canaliculatusen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2020-01-24en_UK
dc.rights.embargoreason[2019-BBA-unformatted.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.bbalip.2019.01.010en_UK
dc.identifier.pmid30684680en_UK
dc.citation.jtitleBiochimica et Biophysica Acta Molecular and Cell Biology of Lipidsen_UK
dc.citation.issn1388-1981en_UK
dc.citation.volume1864en_UK
dc.citation.issue5en_UK
dc.citation.spage619en_UK
dc.citation.epage628en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderNatural Science Foundation of Guangdong ProvinceNatural Science Foundation of Guangdong Provinceen_UK
dc.contributor.funderNational Natural Science Foundation of ChinaNational Natural Science Foundation of Chinaen_UK
dc.contributor.funderNational Natural Science Foundation of ChinaNational Natural Science Foundation of Chinaen_UK
dc.contributor.funderNational Key R&D Program of Chinaen_UK
dc.contributor.funderChina Postdoctoral Science Foundationen_UK
dc.author.emaild.r.tocher@stir.ac.uken_UK
dc.citation.date23/01/2019en_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationSouth China Agricultural Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationSouth China Agricultural Universityen_UK
dc.identifier.isiWOS:000463123300002en_UK
dc.identifier.scopusid2-s2.0-85061112939en_UK
dc.identifier.wtid1100693en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted2019-01-20en_UK
dc.date.filedepositdate2019-01-29en_UK
Appears in Collections:Aquaculture Journal Articles

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