Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Tributyltin is a potent inhibitor of piscine peroxisome proliferator-activated receptor α and β
Author(s): Colliar, Louise
Sturm, Armin
Leaver, Michael
Contact Email:
Keywords: Tributyltin
Water Pollution
Pollution Environmental aspects.
Endocrine toxicology
Issue Date: Jan-2011
Date Deposited: 6-Apr-2011
Citation: Colliar L, Sturm A & Leaver M (2011) Tributyltin is a potent inhibitor of piscine peroxisome proliferator-activated receptor α and β. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 153 (1), pp. 168-173.
Abstract: Increasing evidence suggests that common environmental contaminants can act as endocrine disrupters in fish. However, current data are biased towards environmental estrogens, highlighting the need to elucidate potential pollutant impact on other endocrine axes. Here, we report a highthroughput assay to identify chemicals interacting with piscine peroxisome proliferator-activated receptors (PPARs). Our transactivation assay employs a fish cell line and uses recombinant proteins combining the yeast Gal4 DNA-binding domain with the ligand-binding domain of PPARs from plaice. Compared to assays with full-length PPARs, this approach circumvents interaction of chemicals binding to retinoid X receptors, which form heterodimers with PPAR and many other nuclear receptors. Plaice PPARa and PPARb are activated by fibrate drugs and by phthalate monoesters at concentrations similar to those activating the homologous mammalian receptors. In line with their assumed role as central transcriptional regulators of energy homostasis, a number of fatty acids activate plaice PPARa and PPARb. In contrast, tributyl tin oxide (TBTO) is a potent antagonist of PPARa and PPARb, showing activity at environmentally relevant concentrations of TBTO (1-50 nM). Given the ubiquitous and persistent nature of TBTO, the possibility that chronic environmental effects are occurring via disruption of PPAR signalling in fish should be further investigated. Keywords: tributyltin, TBTO, PPAR, pollutant, fibr
DOI Link: 10.1016/j.cbpc.2010.10.008
Rights: Published in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology by Elsevier

Files in This Item:
File Description SizeFormat 
Colliar2011storre.pdfFulltext - Accepted Version911.41 kBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.