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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Ontogeny of the circadian system during embryogenesis in rainbow trout (Oncorhynchus mykiss) and the effect of prolonged exposure to continuous illumination on daily rhythms of per1, clock, and aanat2 expression
Author(s): Davie, Andrew
Sanchez, Jose A
Vera, LM
Sanchez-Vazquez, F Javier
Migaud, Herve
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Keywords: per1
rainbow trout
circadian rhythms
Issue Date: Apr-2011
Publisher: Informa Healthcare
Citation: Davie A, Sanchez JA, Vera L, Sanchez-Vazquez FJ & Migaud H (2011) Ontogeny of the circadian system during embryogenesis in rainbow trout (Oncorhynchus mykiss) and the effect of prolonged exposure to continuous illumination on daily rhythms of per1, clock, and aanat2 expression, Chronobiology International, 28 (3), pp. 177-186.
Abstract: It is widely held that the development of the circadian system during embryogenesis is important for future survival of an organism. Work in teleosts has been, to date, limited to zebrafish, which provides little insight into the diversity of this system within such a large vertebrate class. In this study, we analyzed the diel expression of per1, clock, and aanat2 in unfertilized rainbow trout oocytes and embryos maintained under either a 12:12 light:dark (LD) cycle or continuous illumination (LL) from fertilization. 24-h profiles in expression were measured at fertilization as well as 8, 21 42, and 57 days postfertilization (dpf). Both per1 and clock were expressed in unfertilized oocytes and all embryonic stages, while aanat2 expression was only measureable from 8 dpf. A reduction in both per1 and clock mean expression level between unfertilized oocytes/0-1dpf embryos and 8-9dpf embryos was suggestive of a transition from maternal RNA to endogenous mRNA expression. While aanat2 expression was not clearly associated with photic conditions, photoperiod treatment did alter the expression of per1 and clock expression/rhythmicity from as early as 8 dpf (per1), which could suggest the presence and functionality of an as yet unidentified “photoreceptor”. As a whole, this work demonstrates that clock systems are present and functional during embryonic development in rainbow trout. Further studies of their expression and regulation will help understand how the environment interacts with embryonic development in the species.
Type: Journal Article
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Rights: This item is embargoed until 31st April 2012, until then please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.; This is an electronic version of an article published in Chronobiology International, 28(3). Chronobiology International is available online at:
Affiliation: Aquaculture
University of Murcia
University of Murcia

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