Fatty acid metabolism in Atlantic salmon (Salmo salar L.) hepatocytes and influence of dietary vegetable oil

Abstract

Isolated hepatocytes from Atlantic salmon (Salmo salar), fed diets containing either 100% fish oil or a vegetable oil blend replacing 75% of the fish oil, were incubated with a range of seven 14C-labelled fatty acids. The fatty acids were; [1-14C]16:0, [1-14C]18:1n-9, [1-14C]18:2n-6, [1-14C]18:3n-3, [1-14C]20:4n-6, [1-14C]20:5n-3, and [1-14C]22:6n-3. After 2 hours of incubation the hepatocytes and medium were analyzed for acid soluble products, incorporation into lipid classes, and hepatocytes for desaturation and elongation. Uptake into hepatocytes was highest with [1-14C]18:2n-6 and [1-14C]20:5n-3 and lowest with [1-14C]16:0. The highest recovery of radioactivity in the cells was found in triacylglycerols. Of the phospholipids the highest recovery was found in phosphatidylcholine, with [1-14C]16:0 and [1-14C]22:6n-3 being the most prominent fatty acids. The rates of β-oxidation were as follows: 20:4n-6 > 18:2n-6 = 16:0 > 18:1n-9 > 22:6n-3 = 18:3n-3 = 20:5n-3. Of the fatty acids taken up by the hepatocytes, [1-14C]16:0 and [1-14C]18:1n-9 were subsequently exported the most, with the majority of radioactivity recovered in phospholipids and triacylglycerols, respectively. The major products from desaturation and elongation were generally one cycle of elongation of the fatty acids. Diet had a clear effect on the overall lipid metabolism, with replacing 75% of the fish oil with vegetable oil resulting in decreased uptake of all fatty acids and reduced incorporation of fatty acids into cellular lipids, but increased β-oxidation activity, and higher recovery in products of desaturation and elongation of [1-14C]18:2n-6 and [1-14C]18:3n-3.