Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/2805
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dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorRotllant, Josepen_UK
dc.contributor.authorCerda-Reverter, Jose Men_UK
dc.contributor.authorDick, James Ren_UK
dc.contributor.authorFigueras, Antonioen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2014-03-17T23:12:57Z-
dc.date.available2014-03-17T23:12:57Z-
dc.date.issued2010-10en_UK
dc.identifier.urihttp://hdl.handle.net/1893/2805-
dc.description.abstractElovl4 is a fatty acyl elongase that participates in the biosynthesis of very long-chain fatty acids (≥C24), which are relatively abundant in skin (saturated chains), or retina, brain and testes (polyunsaturated chains) of mammals. In the present study we characterised two Elovl4 proteins, Elovl4a and Elovl4b, from zebrafish Danio rerio, and investigated their expression patterns during embryonic development. Heterologous expression in baker’s yeast showed that both zebrafish Elovl4 proteins efficiently elongated saturated fatty acids up to C36, with 26:0 appearing the preferred substrate as reported for human ELOVL4. Interestingly, activity for the elongation of PUFA substrates was only shown by Elovl4b, which effectively converted eicosapentaenoic (20:5n-3) and arachidonic (20:4n-6) acids to elongated polyenoic products up to C36. Furthermore, zebrafish Elovl4b may be involved in the biosynthesis of docosahexaenoic acid (22:6n-3, DHA) as it had the capacity to elongate 22:5n-3 to 24:5n-3 which can be subsequently desaturated and chain shortened to DHA in peroxisomes. The distinct functional roles of zebrafish Elovl4 proteins were also reflected in their spatial-temporal expression patterns during ontogeny. Analyses by whole-mount in situ hybridisation in zebrafish embryos showed that elovl4a was expressed in neuronal tissues (wide-spread distribution in the head area), with elovl4b specifically expressed in epiphysis (pineal gland) and photoreceptor cells in the retina. Similarly, tissue distribution in adults revealed that elovl4a transcripts were found in most tissues analysed, whereas elovl4b expression was essentially restricted to eye and gonads. Overall, the results suggest that zebrafish elovl4b resembles other mammalian orthologues in terms of function and expression patterns, whereas elovl4a may represent an alternative elongase not previously described in vertebrates.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMonroig O, Rotllant J, Cerda-Reverter JM, Dick JR, Figueras A & Tocher DR (2010) Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development. Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1801 (10), pp. 1145-1154. http://www.sciencedirect.com/science/journal/13881981; https://doi.org/10.1016/j.bbalip.2010.06.005en_UK
dc.rightsPublished in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids by Elsevier. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1801, Issue 10, October 2010, pp. 1145 - 1154; This is the peer reviewed version of this article.; NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, VOL 1801, ISSUE 10, October 2010. DOI 10.1016/j.bbalip.2010.06.005en_UK
dc.subjectlong-chain polyunsaturated fatty acidsen_UK
dc.subjectelovl4 elongaseen_UK
dc.subjectzebrafishen_UK
dc.subjectDanio rerioen_UK
dc.subjectembryonic developmenten_UK
dc.subjectwhole-mount in situ hybridisationen_UK
dc.subjectgene expressionen_UK
dc.subjectvery long chain fatty acidsen_UK
dc.subjectLipoproteins Fishen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectFish oilsen_UK
dc.titleExpression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic developmenten_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.bbalip.2010.06.005en_UK
dc.citation.jtitleBiochimica et Biophysica Acta Molecular and Cell Biology of Lipidsen_UK
dc.citation.issn1388-1981en_UK
dc.citation.volume1801en_UK
dc.citation.issue10en_UK
dc.citation.spage1145en_UK
dc.citation.epage1154en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.identifier.urlhttp://www.sciencedirect.com/science/journal/13881981en_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.citation.date01/07/2010en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationSpanish National Research Council (CSIC)en_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationSpanish National Research Council (CSIC)en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000281265600005en_UK
dc.identifier.scopusid2-s2.0-77955517699en_UK
dc.identifier.wtid835839en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2010-07-01en_UK
dc.date.filedepositdate2011-03-16en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorRotllant, Josep|en_UK
local.rioxx.authorCerda-Reverter, Jose M|en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.authorFigueras, Antonio|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2011-03-16en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2011-03-16|en_UK
local.rioxx.filenameZebrafish elovl4 final.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1388-1981en_UK
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