Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/28001
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dc.contributor.authorHorvath, Hajnalkaen_UK
dc.contributor.authorKovacs, Attila Wen_UK
dc.contributor.authorRiddick, Caitlinen_UK
dc.contributor.authorPresing, Matyasen_UK
dc.date.accessioned2018-10-19T00:00:54Z-
dc.date.available2018-10-19T00:00:54Z-
dc.date.issued2013-12-31en_UK
dc.identifier.urihttp://hdl.handle.net/1893/28001-
dc.description.abstractPhycocyanin (PC) is one of the water-soluble accessory pigments of cyanobacteria species, and its concentration in aquatic systems is used to estimate the presence and relative abundance of blue-green algae. PC concentration and the PC/Chl-a ratio of four N2-fixing filamentous cyanobacteria strains (Cylindrospermopsis raciborskii, Anabaena spiroides, Aphanizomenon flos-aquae and Aphanizomenon issatschenkoi) common to Lake Balaton (Hungary) were determined using repeated freezing and thawing. A strong linear correlation was found between the extracted PC and Chl-a concentrations for all strains at high Chl-a concentrations (almost stable PC/Chl-a ratio in the range of 20−100 µg l−1 Chl-a). Extraction of PC and Chl-a from samples with low biomass of cyanobacteria (less than 20 µg l−1 Chl-a) proved to be unreliable using the standard protocol of freeze–thaw cycles (coefficients of variation exceeding 10–15%). In order to find an extraction method that is robust in fresh waters characterized by low algae biomass (e.g. Lake Balaton), the effectiveness of four extraction methods (repeated freeze–thaw method and homogenization with mortar and pestle, Ultrasonic, and Polytron homogenizer) were compared using C. raciborskii. It was found that the efficiency of extraction of phycocyanin was highest when a single freeze–thaw cycle was followed by sonication (25% additional yield compared with using the freeze–thaw method alone). Applying this combined method to surface water samples of Lake Balaton, a strong correlation was found between PC concentration and cyanobacterial biomass (R 2 = 0.9436), whilst the repeated freezing–thawing method found no detectable PC content. Here we show that the combined sonication/freeze–thaw method could be suitable for measuring filamentous cyanobacteria PC content, even at low concentrations; as well as for the estimation of cyanobacterial contribution to total biomass in fresh waters.en_UK
dc.language.isoenen_UK
dc.publisherTaylor and Francisen_UK
dc.relationHorvath H, Kovacs AW, Riddick C & Presing M (2013) Extraction methods for phycocyanin determination in freshwater filamentous cyanobacteria and their application in a shallow lake. European Journal of Phycology, 48 (3), pp. 278-286. https://doi.org/10.1080/09670262.2013.821525.en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectCylindrospermopsis raciborskiien_UK
dc.subjectextraction methodsen_UK
dc.subjectfresh wateren_UK
dc.subjectN2-fixing filamentous cyanobacteriaen_UK
dc.subjectphycocyaninen_UK
dc.subjectpigment analysesen_UK
dc.titleExtraction methods for phycocyanin determination in freshwater filamentous cyanobacteria and their application in a shallow lakeen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[Horvath et al 2013 EJP.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1080/09670262.2013.821525en_UK
dc.citation.jtitleEuropean Journal of Phycologyen_UK
dc.citation.issn1469-4433en_UK
dc.citation.issn0967-0262en_UK
dc.citation.volume48en_UK
dc.citation.issue3en_UK
dc.citation.spage278en_UK
dc.citation.epage286en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderThe Carnegie Trusten_UK
dc.author.emailcaitlin.riddick1@stir.ac.uken_UK
dc.citation.date23/07/2013en_UK
dc.contributor.affiliationHungarian Academy of Sciencesen_UK
dc.contributor.affiliationHungarian Academy of Sciencesen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationHungarian Academy of Sciencesen_UK
dc.identifier.isi000324367000004en_UK
dc.identifier.scopusid2-s2.0-84884485258en_UK
dc.identifier.wtid875574en_UK
dc.contributor.orcid0000-0002-5694-2377en_UK
dc.date.accepted2013-01-07en_UK
Appears in Collections:Biological and Environmental Sciences Journal Articles

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