Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27953
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Metformin attenuates lipid accumulation in hepatocytes of blunt snout bream (Megalobrama amblycephala) via activation of AMP-activated protein kinase
Author(s): Zhou, Wenhao
Rahimnejad, Samad
Tocher, Douglas R
Lu, Kangle
Zhang, Chunxiao
Sun, Yunzhang
Contact Email: d.r.tocher@stir.ac.uk
Keywords: Blunt snout bream
Fatty liver
Metformin
AMP-activated protein kinase
Lipid metabolism
Issue Date: 15-Jan-2019
Citation: Zhou W, Rahimnejad S, Tocher DR, Lu K, Zhang C & Sun Y (2019) Metformin attenuates lipid accumulation in hepatocytes of blunt snout bream (Megalobrama amblycephala) via activation of AMP-activated protein kinase. Aquaculture, 499, pp. 90-100. https://doi.org/10.1016/j.aquaculture.2018.09.028
Abstract: Currently, there is a trend to use high-fat diets in intensive aquaculture that is accompanied with incidence of fatty liver when dietary lipid level surpasses an upper limit. So, it is necessary to develop appropriate strategies to reduce the risk of fatty liver in commercial fish farming. Studies in mammals have revealed a correlation between fatty liver and AMP-activated protein kinase (AMPK) activity, which has been recognized as a key modulator of lipid metabolism. Considering the frequent occurrence of fatty liver in blunt snout bream farming, an in-vitro study was designed to evaluate the efficiency of metformin, as a stimulator of AMPK, in activation of AMPK and its subsequent effects on lipid metabolism in primary hepatocytes. Fish hepatocytes were seeded at a density of 1 × 106 ml−1 in 6-well tissue culture plates and treated with three different media including: 1) Leibovitz's L-15 medium [L15] as control, 2) high-fat medium [L15 + 400 μM oleic acid], and 3) metformin medium [L15 + 400 μM oleic acid+200 μM metformin]. After 48 h of culture, the cells and supernatant were collected for analysis. The results showed significant (P 
DOI Link: 10.1016/j.aquaculture.2018.09.028
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Zhou W, Rahimnejad S, Tocher DR, Lu K, Zhang C & Sun Y (2019) Metformin attenuates lipid accumulation in hepatocytes of blunt snout bream (Megalobrama amblycephala) via activation of AMP-activated protein kinase. Aquaculture, 499, pp. 90-100. DOI: https://doi.org/10.1016/j.aquaculture.2018.09.028 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Licence URL(s): http://creativecommons.org/licenses/by-nc-nd/4.0/

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