Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27629
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dc.contributor.authorUsher, Sarahen_UK
dc.contributor.authorHan, Lihuaen_UK
dc.contributor.authorHaslam, Richard Pen_UK
dc.contributor.authorMichaelson, Louise Ven_UK
dc.contributor.authorSturtevant, Drewen_UK
dc.contributor.authorAziz, Minaen_UK
dc.contributor.authorChapman, Kent Den_UK
dc.contributor.authorSayanova, Olgaen_UK
dc.contributor.authorNapier, Johnathan A.en_UK
dc.date.accessioned2018-08-17T00:03:33Z-
dc.date.available2018-08-17T00:03:33Z-
dc.date.issued2017-12-31en_UK
dc.identifier.other6570en_UK
dc.identifier.urihttp://hdl.handle.net/1893/27629-
dc.description.abstractThere is considerable interest in the de novo production of omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), not least of all given the importance of these fatty acids in both aquaculture and human nutrition. Previously we have demonstrated the feasibility of using metabolic engineering in transgenic plants (Camelina sativa) to modify the seed oil composition to now include EPA and/or DHA. In this study, we further tailored the seed oil profile to reduce the omega-6 content, and evaluated the performance of such GM plants under field conditions (i.e. environmental releases), in terms of agronomic performance and also the lipidomic profile of seed oil. We used MALDI- mass spectrometry imaging to identify discrete tissue-types in the seed in which these non-native fatty acids preferentially accumulated. Collectively, these data provide new insights into the complexity of plant lipid metabolism and the challenges associated with predictive manipulation of these pathways. However, this study identified the likely dispensable nature of a Δ12-desturase activity in our omega-3 metabolic engineering rationales for Camelina.en_UK
dc.language.isoenen_UK
dc.publisherSpringer Natureen_UK
dc.relationUsher S, Han L, Haslam RP, Michaelson LV, Sturtevant D, Aziz M, Chapman KD, Sayanova O & Napier JA (2017) Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa. Scientific Reports, 7 (1), Art. No.: 6570. https://doi.org/10.1038/s41598-017-06838-0en_UK
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_UK
dc.titleTailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativaen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1038/s41598-017-06838-0en_UK
dc.citation.jtitleScientific Reportsen_UK
dc.citation.issn2045-2322en_UK
dc.citation.volume7en_UK
dc.citation.issue1en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen_UK
dc.citation.date26/07/2017en_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.contributor.affiliationUniversity of North Texasen_UK
dc.contributor.affiliationUniversity of North Texasen_UK
dc.contributor.affiliationUniversity of North Texasen_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.identifier.wtid954608en_UK
dc.contributor.orcid0000-0001-5621-4495en_UK
dc.contributor.orcid0000-0003-3580-3607en_UK
dc.date.accepted2017-06-12en_UK
dc.description.refREF Eligible with Permitted Exceptionen_UK
dc.date.filedepositdate2018-08-16en_UK
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