Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27585
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Globular-shaped variable lymphocyte receptors B antibody multimerized by a hydrophobic clustering in hagfish
Author(s): Kim, Jaesung
Im, Se Pyeong
Lee, Jung Seok
Lazarte, Jassy Mary S
Kim, Si Won
Jung, Jae Wook
Kim, Jong Yong
Kim, Young Rim
Lee, Sangmin
Kim, Gwang Joong
Jung, Hyun Suk
Lee, Kyun Oh
Adams, Alexandra
Thompson, Kim D
Jung, Tae Sung
Issue Date: 31-Dec-2018
Citation: Kim J, Im SP, Lee JS, Lazarte JMS, Kim SW, Jung JW, Kim JY, Kim YR, Lee S, Kim GJ, Jung HS, Lee KO, Adams A, Thompson KD & Jung TS (2018) Globular-shaped variable lymphocyte receptors B antibody multimerized by a hydrophobic clustering in hagfish, Scientific Reports, 8, Art. No.: 10801. https://doi.org/10.1038/s41598-018-29197-w.
Abstract: In hagfish and lampreys, two representative jawless vertebrates, the humoral immunity is directly mediated by variable lymphocyte receptors B (VLRBs). Both monomeric VLRBs are structurally and functionally similar, but their C-terminal tails differ: lamprey VLRB has a Cys-rich tail that forms disulfide-linked pentamers of dimers, contributing to its multivalency, whereas hagfish VLRB has a superhydrophobic tail of unknown structure. Here, we reveal that VLRBs obtained from hagfish plasma have a globular-shaped multimerized form (approximately 0.6 to 1.7 MDa) that is generated by hydrophobic clustering instead of covalent linkage. Electron microscopy (EM) and single-particle analysis showed that the multimerized VLRBs form globular-shaped clusters with an average diameter of 28.7 ± 2.2 nm. The presence of VLRBs in the complex was confirmed by immune-EM analysis using an anti-VLRB antibody. Furthermore, the hydrophobic hagfish C-terminus (HC) was capable of triggering multimerization and directing the cellular surface localization via a glycophosphatidylinositol linkage. Our results strongly suggest that the hagfish VLRB forms a previously unknown globular-shaped antibody. This novel identification of a structurally unusual VLRB complex may suggest that the adaptive immune system of hagfish differs from that of lamprey.
DOI Link: 10.1038/s41598-018-29197-w
Rights: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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