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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: New diagnostic SNP molecular markers for the Mytilus species complex
Author(s): Wilson, Joanna
Matejusova, Iveta
McIntosh, Rebecca E
Carboni, Stefano
Bekaert, Michaël
Keywords: General Biochemistry
Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Medicine
Issue Date: 12-Jul-2018
Citation: Wilson J, Matejusova I, McIntosh RE, Carboni S & Bekaert M (2018) New diagnostic SNP molecular markers for the Mytilus species complex, PLOS ONE, 13 (7), Art. No.: e0200654.
Abstract: The development of diagnostic markers has been a long-standing interest of population geneticists as it allows clarification of taxonomic uncertainties. Historically, there has been much debate on the taxonomic status of species belonging to the Mytilus species complex (M. edulis, M. galloprovincialis and M. trossulus), and whether they are discrete species. We analysed reference pure specimens of M. edulis, M. galloprovincialis and M. trossulus, using Restriction site associated DNA (RAD) sequencing and identified over 6,000 SNP markers separating the three species unambiguously. We developed a panel of diagnostic SNP markers for the genotyping of Mytilus species complex as well as the identification of hybrids and interspecies introgression events in Mytilus species. We validated a panel of twelve diagnostic SNP markers which can be used for species genotyping. Being able to accurately identify species and hybrids within the Mytilus species complex is important for the selective mussel stock management, the exclusion of invasive species, basic physiology and bio-diversity studies.
DOI Link: 10.1371/journal.pone.0200654
Rights: © 2018 Wilson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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