Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27462
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dc.contributor.authorHuyben, Daviden_UK
dc.contributor.authorNyman, Andreasen_UK
dc.contributor.authorVidaković, Aleksandaren_UK
dc.contributor.authorPassoth, Volkmaren_UK
dc.contributor.authorMoccia, Richarden_UK
dc.contributor.authorKiessling, Andersen_UK
dc.contributor.authorDicksved, Johanen_UK
dc.contributor.authorLundh, Torbjörnen_UK
dc.date.accessioned2018-06-30T00:02:46Z-
dc.date.available2018-06-30T00:02:46Z-
dc.date.issued2017-04-20en_UK
dc.identifier.urihttp://hdl.handle.net/1893/27462-
dc.description.abstractRainbow trout (Oncorhynchus mykiss) were fed for 10 weeks on a diet containing either 30% fish meal (FM) or with 20, 40 and 60% replacement of fish meal protein with Saccharomyces cerevisiae (SC) or a mixture of Wickerhamomyces anomalus and S. cerevisiae (WA). Luminal contents and mucosal tissue from the distal intestine were collected and analysed for yeast and bacterial loads by agar plating. Diversity and abundance were determined by sequencing of amplicons generated from the 26S rRNA (yeast) and 16S rRNA (bacteria) genes. In addition, the diets were analysed before and after feed extrusion to determine the viability and composition of the yeasts ingested by the fish. After extrusion, 9–10 log cells g− 1 of yeast were still intact in the SC and WA diets, but culturable yeast showed log-reductions of 5–7 CFU g− 1. For yeasts isolated from the gut contents, 81–96% of colonies consisted of Debaryomyces hansenii, with few or no colonies of S. cerevisiae or W. anomalus despite their high inclusion rate in the diets. Characterisation of gut bacteria using Illumina MiSeq showed that 70 and 19% of sequences were classified to the phyla Firmicutes and Proteobacteria, specifically sequences identified as Leuconostocaceae, Lactobacillaceae and Photobacterium. Compared with the FM diet, the WA40 diet reduced bacterial diversity, whereas the WA60 diet increased the abundance of the pathogenic yeast Candida albicans and reduced lactic acid bacteria in the gut. Overall, 40 and 60% replacement of fish meal protein with a mixture of W. anomalus and S. cerevisiae significantly altered the gut microbiota of rainbow trout, while 20% replacement and diets with only S. cerevisiae had little or no effect.en_UK
dc.language.isoenen_UK
dc.publisherElsevier BVen_UK
dc.relationHuyben D, Nyman A, Vidaković A, Passoth V, Moccia R, Kiessling A, Dicksved J & Lundh T (2017) Effects of dietary inclusion of the yeasts Saccharomyces cerevisiae and Wickerhamomyces anomalus on gut microbiota of rainbow trout, Aquaculture, 473, pp. 528-537. https://doi.org/10.1016/j.aquaculture.2017.03.024.en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectRainbow trouten_UK
dc.subjectGut microbiotaen_UK
dc.subjectYeasten_UK
dc.subjectSaccharomyces cerevisiaeen_UK
dc.subjectWickeren_UK
dc.subjecthamomyces anomalusen_UK
dc.subjectIllumina next generation sequencingen_UK
dc.titleEffects of dietary inclusion of the yeasts Saccharomyces cerevisiae and Wickerhamomyces anomalus on gut microbiota of rainbow trouten_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[1-s2.0-S0044848617301680-main.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.aquaculture.2017.03.024en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume473en_UK
dc.citation.spage528en_UK
dc.citation.epage537en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderSvenska Forskningsrådet Formasen_UK
dc.author.emaildavid.huyben@stir.ac.uken_UK
dc.citation.date14/03/2017en_UK
dc.contributor.affiliationSwedish University of Agricultural Sciencesen_UK
dc.contributor.affiliationSwedish University of Agricultural Sciencesen_UK
dc.contributor.affiliationSwedish University of Agricultural Sciencesen_UK
dc.contributor.affiliationSwedish University of Agricultural Sciencesen_UK
dc.contributor.affiliationUniversity of Guelphen_UK
dc.contributor.affiliationSwedish University of Agricultural Sciencesen_UK
dc.contributor.affiliationSwedish University of Agricultural Sciencesen_UK
dc.contributor.affiliationSwedish University of Agricultural Sciencesen_UK
dc.identifier.wtid906141en_UK
dc.contributor.orcid0000-0001-7913-851Xen_UK
dc.date.accepted2017-03-11en_UK
dc.description.refREF Eligible with Permitted Exceptionen_UK
Appears in Collections:Aquaculture Journal Articles

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