|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Characterization of the outer membrane proteome of Francisella noatunensis subsp. orientalis|
Thompson, Kim D
Inglis, Neil F
Ramirez-Paredes, Jose Gustavo
Monaghan, Sean J
|Keywords:||Francisella noatunensis subsp. orientalis|
Outer membrane proteins
|Citation:||Shahin K, Thompson KD, Inglis NF, McLean K, Ramirez-Paredes JG, Monaghan SJ, Hoare R, Fontaine M, Metselaar M & Adams A (2018) Characterization of the outer membrane proteome of Francisella noatunensis subsp. orientalis. Journal of Applied Microbiology, 125 (3), pp. 686-699. https://doi.org/10.1111/jam.13918|
|Abstract:||Aims The aims of the current study were to characterise the outer membrane proteins (OMPs) of Francisella noatunensis subsp. orientalis (Fno) STIR‐GUS‐F2f7, and identify proteins recognised by sera from tilapia, Oreochromis niloticus, (L) that survived experimental challenge with Fno. Methods and Results The composition of the OMPs of a virulent strain of Fno (STIR‐GUS‐ F2f7), isolated from diseased red Nile tilapia in UK, was examined. The sarcosine‐insoluble OMP fraction was screened with tilapia hyper‐immune sera by western blot analysis following separation of the proteins by 1D SDS‐PAGE. Liquid chromatography‐electrospray ionisation‐tandem mass spectrometry (LC‐ESI‐MS/MS) was used to identify the various proteins present in the OMP profile. Two hundred and thirty‐nine proteins were identified, of which 44 were found in the immunogenic band recognised by the tilapia hyperimmune serum. In silico analysis was performed to predict the function and location of the OMPs identified by MS. Conclusions Using a powerful proteomic‐based approach in conjugation with western immunoblotting, proteins comprising the outer membrane fraction of Fno STIR‐GUS‐F2f7 were identified, catalogued and screened for immune recognition by tilapia sera. Significance and Impact of the study The current study is the first report on the characterisation of Fno OMPs. The findings here provide preliminary data on bacterial surface proteins that exist in direct contact with the host's immune defences during infection and offer an insight into the pathogenesis of Fno.|
|Rights:||This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Shahin, K., Thompson, K., Inglis, N., Mclean, K., Ramirez‐Paredes, J., Monaghan, S., Hoare, R., Fontaine, M., Metselaar, M. and Adams, A. (2018), Characterization of the outer membrane proteome of Francisella noatunensis subsp. orientalis . J Appl Microbiol, 125: 686-699, which has been published in final form at https://doi.org/10.1111/jam.13918. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving.|
|Shahin_et_al-2018-Journal_of_Applied_Microbiology.pdf||Fulltext - Accepted Version||1.53 MB||Adobe PDF||View/Open|
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
If you believe that any material held in STORRE infringes copyright, please contact email@example.com providing details and we will remove the Work from public display in STORRE and investigate your claim.