Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26854
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Functional evidence for the inflammatory reflex in teleosts: A novel alpha7 nicotinic acetylcholine receptor modulates the macrophage response to dsRNA
Author(s): Torrealba, Debora
Balasch, Joan C
Criado, Manuel
Tort, Lluis
MacKenzie, Simon
Roher, Nerea
Contact Email: simon.mackenzie@stir.ac.uk
Keywords: α7nAChR
Nicotine
Poly(I:C)
Innate immunity
Evolution
Issue Date: 31-Jul-2018
Citation: Torrealba D, Balasch JC, Criado M, Tort L, MacKenzie S & Roher N (2018) Functional evidence for the inflammatory reflex in teleosts: A novel alpha7 nicotinic acetylcholine receptor modulates the macrophage response to dsRNA, Developmental and Comparative Immunology, 84, pp. 279-291. https://doi.org/10.1016/j.dci.2018.02.020.
Abstract: The inflammatory reflex modulates the innate immune system, keeping in check the detrimental consequences of overstimulation. A key player controlling the inflammatory reflex is the alpha 7 acetylcholine receptor (α7nAChR). This receptor is one of the signalling molecules regulating cytokine expression in macrophages. In this study, we characterize a novel teleost α7nAChR. Protein sequence analysis shows a high degree of conservation with mammalian orthologs and trout α7nAChR has all the features and essential amino acids to form a fully functional receptor. We demonstrate that trout macrophages can bind α-bungarotoxin (α-BTX), a competitive antagonist for α7nAChRs. Moreover, nicotine stimulation produces a decrease in pro-inflammatory cytokine expression after stimulation with poly(I:C). These results suggest the presence of a functional α7nAChR in the macrophage plasma membrane. Further, in vivo injection of poly(I:C) induced an increase in serum ACh levels in rainbow trout. Our results manifest for the first time the functional conservation of the inflammatory reflex in teleosts.
DOI Link: 10.1016/j.dci.2018.02.020
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Torrealba D, Balasch JC, Criado M, Tort L, MacKenzie S & Roher N (2018) Functional evidence for the inflammatory reflex in teleosts: A novel alpha7 nicotinic acetylcholine receptor modulates the macrophage response to dsRNA, Developmental and Comparative Immunology, 84, pp. 279-291. DOI: 10.1016/j.dci.2018.02.020 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

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