Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26726
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dc.contributor.authorYuan, Xiaofei-
dc.contributor.authorSong, Yanqing-
dc.contributor.authorSong, Yizhi-
dc.contributor.authorXu, Jiabao-
dc.contributor.authorWu, Yinhu-
dc.contributor.authorGlidle, Andrew-
dc.contributor.authorCusack, Maggie-
dc.contributor.authorIjaz, Umer Z-
dc.contributor.authorCooper, Jonathan M-
dc.contributor.authorHuang, Wei E-
dc.contributor.authorYin, Huabing-
dc.date.accessioned2018-05-04T00:43:29Z-
dc.date.available2018-05-04T00:43:29Z-
dc.date.issued2018-04-
dc.identifier.othere02508-17-
dc.identifier.urihttp://hdl.handle.net/1893/26726-
dc.description.abstractLasers are instrumental in advanced bioimaging and Raman spectroscopy. However, they are also well known for their destructive effects on living organisms, leading to concerns about the adverse effects of laser technologies. To implement Raman spectroscopy for cell analysis and manipulation, such as Raman activated cell sorting, it is crucial to identify non-destructive conditions for living cells. Here, we evaluated quantitatively the effect of 532 nm laser irradiation on bacterial cell fate and growth at the single-cell level. Using a purpose-built microfluidic platform, we were able to quantify the growth characteristics i.e. specific growth rate and lag time of individual cells as well as the survival rate of a population in conjunction with Raman spectroscopy. Representative Gram-negative and Gram-positive species show a similar trend in response to laser irradiation dose. Laser irradiation could compromise physiological function of cells and the degree of destruction is both dose and strain dependent, ranging from reduced cell growth to a complete loss of cell metabolic activity and finally to physical disintegration. Gram-positive bacterial cells are more susceptible than Gram-negative bacterial strains to irradiation-induced damage. By directly correlating Raman acquisition with single cell growth characteristics, we provide evidence of non-destructive characteristics of Raman spectroscopy on individual bacterial cells. However, while strong Raman signals can be obtained without causing cell death, the variety of responses from different strains and from individual cells justify careful evaluation of Raman acquisition conditions if cell viability is critical.en_UK
dc.language.isoen-
dc.publisherAmerican Society for Microbiology-
dc.relationYuan X, Song Y, Song Y, Xu J, Wu Y, Glidle A, Cusack M, Ijaz UZ, Cooper JM, Huang WE & Yin H (2018) Effect of laser irradiation on cell function and its implications in Raman spectroscopy, Applied and Environmental Microbiology, 84 (8), Art. No.: e02508-17.-
dc.rightsCopyright © 2018 Yuan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/).-
dc.subjectsingle cellen_UK
dc.subjectmicrofluidicsen_UK
dc.subjectRaman spectroscopyen_UK
dc.subjectviabilityen_UK
dc.subjectlaseren_UK
dc.subjectgrowthen_UK
dc.subjectRaman activated cell sorting (RACS)en_UK
dc.titleEffect of laser irradiation on cell function and its implications in Raman spectroscopyen_UK
dc.typeJournal Articleen_UK
dc.identifier.doihttp://dx.doi.org/10.1128/aem.02508-17-
dc.identifier.pmid29427427-
dc.citation.jtitleApplied and Environmental Microbiology-
dc.citation.issn0099-2240-
dc.citation.volume84-
dc.citation.issue8-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.citation.date02/02/2018-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationUniversity of Oxford-
dc.contributor.affiliationUniversity of Oxford-
dc.contributor.affiliationUniversity of Oxford-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationFaculty of Natural Sciences-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationUniversity of Oxford-
dc.contributor.affiliationUniversity of Glasgow-
dc.identifier.isi000429665700009-
Appears in Collections:Biological and Environmental Sciences Journal Articles

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