Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26723
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dc.contributor.authorPang, Huanyingen_UK
dc.contributor.authorQiu, Mingshengen_UK
dc.contributor.authorZhao, Jingminen_UK
dc.contributor.authorHoare, Rowenaen_UK
dc.contributor.authorMonaghan, Seanen_UK
dc.contributor.authorSong, Daweien_UK
dc.contributor.authorChang, Yunshengen_UK
dc.contributor.authorJian, Jichangen_UK
dc.date.accessioned2018-04-11T09:35:21Z-
dc.date.availablenull-
dc.date.issued2018-05en_UK
dc.identifier.urihttp://hdl.handle.net/1893/26723-
dc.description.abstractVibrio alginolyticus, a bacterial pathogen in fish and humans, expresses a type III secretion system (T3SS) that is critical for pathogen virulence and disease development. However, little is known about the associated effectors (T3SEs) and their physiological role. In this study, the T3SE gene hopPmaJ (hop) was cloned from V. alginolyticus wild-type strain HY9901 and the mutant strain HY9901Δhop was constructed by the in-frame deletion method. The results showed that the deduced amino acid sequence of V. alginolyticus HopPmaJ shared 78–98% homology with other Vibrio spp. In addition, the HY9901Δhop mutant showed an attenuated swarming phenotype and a 2600-fold decrease in the virulence to grouper. However, the HY9901Δhop mutant showed no difference in morphology, growth, biofilm formation and ECPase activity. Finally, grouper vaccinated via intraperitoneal (IP) injection with HY9901Δhop induced a high antibody titer with a relative percent survival (RPS) value of 84% after challenging with the wild-type HY9901. Real-time PCR assays showed that vaccination with HY9901Δhop enhanced the expression of immune-related genes, including MHC-Iα, MHC-IIα, IgM, and IL-1β after vaccination, indicating that it is able to induce humoral and cell-mediated immune response in grouper. These results demonstrate that the HY9901Δhop mutant could be used as an effective live vaccine to combat V. alginolyticus in grouper.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationPang H, Qiu M, Zhao J, Hoare R, Monaghan S, Song D, Chang Y & Jian J (2018) Construction of a Vibrio alginolyticus hopPmaJ (hop) mutant and evaluation of its potential as a live attenuated vaccine in orange-spotted grouper (Epinephelus coioides), Fish and Shellfish Immunology, 76, pp. 93-100. https://doi.org/10.1016/j.fsi.2018.02.012.en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Pang H, Qiu M, Zhao J, Hoare R, Monaghan S, Song D, Chang Y & Jian J (2018) Construction of a Vibrio alginolyticus hopPmaJ (hop) mutant and evaluation of its potential as a live attenuated vaccine in orange-spotted grouper (Epinephelus coioides), Fish and Shellfish Immunology, 76, pp. 93-100. DOI: 10.1016/j.fsi.2018.02.012 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectVibrio alginolyticusen_UK
dc.subjectT3SSen_UK
dc.subjecthopPmaJen_UK
dc.subjectLive attenuated vaccineen_UK
dc.subjectEpinephelus coioidesen_UK
dc.titleConstruction of a Vibrio alginolyticus hopPmaJ (hop) mutant and evaluation of its potential as a live attenuated vaccine in orange-spotted grouper (Epinephelus coioides)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2019-02-07en_UK
dc.rights.embargoreason[1-s2.0-S1050464818300743-main.pdf] : Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.fsi.2018.02.012en_UK
dc.identifier.pmid29427720en_UK
dc.citation.jtitleFish and Shellfish Immunologyen_UK
dc.citation.issn1050-4648en_UK
dc.citation.volume76en_UK
dc.citation.spage93en_UK
dc.citation.epage100en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailrowena.hoare1@stir.ac.uken_UK
dc.citation.date07/02/2018en_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.identifier.isi000430888400011en_UK
dc.identifier.scopusid2-s2.0-85042701353en_UK
dc.identifier.wtid880081en_UK
dc.contributor.orcid0000-0002-9298-4275en_UK
dc.contributor.orcid0000-0002-7692-7756en_UK
dc.date.accepted2018-02-06en_UK
dc.date.firstcompliantdepositdate2018-02-15en_UK
dc.description.refREF Compliant by Deposit in Stirling's Repositoryen_UK
Appears in Collections:Aquaculture Journal Articles

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