Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26549
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dc.contributor.authorDong, Yeweien_UK
dc.contributor.authorZhao, Jianhongen_UK
dc.contributor.authorChen, Junliangen_UK
dc.contributor.authorWang, Shuqien_UK
dc.contributor.authorLiu, Yangen_UK
dc.contributor.authorZhang, Qinghaoen_UK
dc.contributor.authorYou, Cuihongen_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorLi, Yuanyouen_UK
dc.date.accessioned2018-04-07T04:56:55Z-
dc.date.available2018-04-07T04:56:55Zen_UK
dc.date.issued2018-03-20en_UK
dc.identifier.urihttp://hdl.handle.net/1893/26549-
dc.description.abstractThe rabbitfish Siganus canaliculatus was the first marine teleost demonstrated to have the ability of biosynthesizing long-chain polyunsaturated fatty acids (LC-PUFA) from C18 PUFA precursors, and all genes encoding the key enzymes for LC-PUFA biosynthesis have been cloned and functionally characterized, which provides us a potential model to study the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. As the primary step to clarify such mechanisms, present research focused on promoter analysis of gene encoding ∆6/∆5 fatty acyl desaturase (Fad), a rate-limiting enzyme catalyzing the first step in the conversion of C18 PUFA to LC-PUFA. First, 2044 bp promoter sequence was cloned by genome walking, and the sequence from -456 bp to + 51bp was determined as core promoter by progressive deletion mutation. Moreover, binding sites of transcription factors (TF) such as CCAAT enhancer binding protein (C/EBP), nuclear factor 1 (NF-1), stimulatory protein 1 (Sp1), nuclear factor Y (NF-Y), activated protein 1 (AP1), sterol regulatory element (SRE), hepatocyte nuclear factor 4α (HNF4α) and peroxisome proliferator activated receptor γ (PPARγ) were identified in the core promoter by site-directed mutation and functional assays. Moreover, NF-1 and HNF4α were confirmed to interact with the core promoter region by gel shift assay and mass spectrometry. This is the first report of the promoter structure of a ∆6/∆5 Fad in a marine teleost, and a novel discovery of NF-1 and HNF4α binding to the ∆6/∆5 Fad promoter.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationDong Y, Zhao J, Chen J, Wang S, Liu Y, Zhang Q, You C, Monroig O, Tocher DR & Li Y (2018) Cloning and characterization of ∆6/∆5 fatty acyl desaturase (Fad) gene promoter in the marine teleost Siganus canaliculatus. Gene, 647, pp. 174-180. https://doi.org/10.1016/j.gene.2018.01.031en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Dong Y, Zhao J, Chen J, Wang S, Liu Y, Zhang Q, You C, Monroig O, Tocher DR & Li Y (2018) Cloning and characterization of ∆6/∆5 fatty acyl desaturase (Fad) gene promoter in the marine teleost Siganus canaliculatus , Gene, 647, pp. 174-180. DOI: 10.1016/j.gene.2018.01.031 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectFatty acyl desaturaseen_UK
dc.subjectLC-PUFA biosynthesisen_UK
dc.subjecttranscriptional regulation mechanismen_UK
dc.subjectrabbitfish Siganus canaliculatusen_UK
dc.subjectmarine teleosten_UK
dc.subjectNF-1en_UK
dc.subjectHNF4αen_UK
dc.titleCloning and characterization of ∆6/∆5 fatty acyl desaturase (Fad) gene promoter in the marine teleost Siganus canaliculatusen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2019-01-10en_UK
dc.rights.embargoreason[Dong D6D5 promoter (Gene 2018).pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.gene.2018.01.031en_UK
dc.identifier.pmid29329928en_UK
dc.citation.jtitleGeneen_UK
dc.citation.issn0378-1119en_UK
dc.citation.volume647en_UK
dc.citation.spage174en_UK
dc.citation.epage180en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailoscar.monroig@stir.ac.uken_UK
dc.citation.date09/01/2018en_UK
dc.contributor.affiliationSouth China Agricultural Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationSouth China Agricultural Universityen_UK
dc.identifier.isiWOS:000425576500022en_UK
dc.identifier.scopusid2-s2.0-85041651416en_UK
dc.identifier.wtid505112en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted2018-01-08en_UK
dc.date.filedepositdate2018-01-17en_UK
Appears in Collections:Aquaculture Journal Articles

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