Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26542
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dc.contributor.authorWang, Shuqien_UK
dc.contributor.authorChen, Junliangen_UK
dc.contributor.authorJiang, Danlien_UK
dc.contributor.authorZhang, Qinghaoen_UK
dc.contributor.authorYou, Cuihongen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorDong, Yeweien_UK
dc.contributor.authorLi, Yuanyouen_UK
dc.date.accessioned2018-01-25T13:29:43Z-
dc.date.available2018-01-25T13:29:43Z-
dc.date.issued2018-06-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/26542-
dc.description.abstractLong chain polyunsaturated fatty acid (LC-PUFA) biosynthesis is an important metabolic pathway in vertebrates, especially fish, considering they are the major source of n-3 LC-PUFA in the human diet. However, most fish have only limited capability for biosynthesis of LC-PUFA. The rabbitfish (Siganus canaliculatus) is able to synthesize LC-PUFA as it has all the key enzyme activities required including Δ6Δ5 Fads2, Δ4 Fads2, Elovl5 and Elovl4. We previously reported a direct interaction between the transcription factor Hnf4α and the promoter regions of Δ4 and Δ6Δ5 Fads2, which suggested that Hnf4α was involved in the transcriptional regulation of fads2 in rabbitfish. For further functionally investigating it, a full-length cDNA of 1736 bp encoding rabbitfish Hnf4α with 454 amino acids was cloned, which was highly expressed in intestine, followed by liver and eyes. Similar to the expression characteristics of its target genes Δ4 and Δ6Δ5 fads2, levels of hnf4α mRNA in liver and eyes were higher in fish reared at low salinity than those reared in high salinity. After the rabbitfish primary hepatocytes were respectively incubated with Alverine, Benfluorex or BI6015, which were anticipated agonists or antagonist for Hnf4α, the mRNA level of Δ6Δ5 and Δ4 fads2 displayed a similar change tendency with that of hnf4α mRNA. Furthermore, when the mRNA level of hhf4α was knocked down using siRNA, the expression of Δ6Δ5 and Δ4 fads2 also decreased. Together, these data suggest that Hnf4α is involved in the transcriptional regulation of LC-PUFA biosynthesis, specifically, by targeting Δ4 and Δ6Δ5 fads2 in rabbitfish.en_UK
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.relationWang S, Chen J, Jiang D, Zhang Q, You C, Tocher DR, Monroig O, Dong Y & Li Y (2018) Hnf4α is involved in the regulation of vertebrate LC-PUFA biosynthesis: insights into the regulatory role of Hnf4α on expression of liver fatty acyl desaturases in the marine teleost Siganus canaliculatus. Fish Physiology and Biochemistry, 44 (3), pp. 805-815. https://doi.org/10.1007/s10695-018-0470-8en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is a post-peer-review, pre-copyedit version of an article published in Fish Physiology and Biochemistry. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10695-018-0470-8en_UK
dc.subjectfatty acyl desaturaseen_UK
dc.subjectLC-PUFA biosynthesisen_UK
dc.subjectHnf4αen_UK
dc.subjecttranscriptional regulation mechanismen_UK
dc.subjectrabbitfish Siganus canaliculatusen_UK
dc.titleHnf4α is involved in the regulation of vertebrate LC-PUFA biosynthesis: insights into the regulatory role of Hnf4α on expression of liver fatty acyl desaturases in the marine teleost Siganus canaliculatusen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2019-06-02en_UK
dc.rights.embargoreason[MS_FISH-D-17-00276.pdf] Until this work is formally published there will be an embargo on the full text of this work.en_UK
dc.identifier.doi10.1007/s10695-018-0470-8en_UK
dc.identifier.pmid29352428en_UK
dc.citation.jtitleFish Physiology and Biochemistryen_UK
dc.citation.issn1573-5168en_UK
dc.citation.issn0920-1742en_UK
dc.citation.volume44en_UK
dc.citation.issue3en_UK
dc.citation.spage805en_UK
dc.citation.epage815en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailoscar.monroig@stir.ac.uken_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationSouth China Agricultural Universityen_UK
dc.identifier.isiWOS:000432520500004en_UK
dc.identifier.scopusid2-s2.0-85040682749en_UK
dc.identifier.wtid505141en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.date.accepted2018-01-11en_UK
dcterms.dateAccepted2018-01-11en_UK
dc.date.filedepositdate2018-01-17en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorWang, Shuqi|en_UK
local.rioxx.authorChen, Junliang|en_UK
local.rioxx.authorJiang, Danli|en_UK
local.rioxx.authorZhang, Qinghao|en_UK
local.rioxx.authorYou, Cuihong|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorDong, Yewei|en_UK
local.rioxx.authorLi, Yuanyou|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2019-06-02en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2019-06-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2019-06-02|en_UK
local.rioxx.filenameMS_FISH-D-17-00276.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0920-1742en_UK
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