Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26181
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dc.contributor.authorAnderson, Craigen_UK
dc.contributor.authorCunha, Luisen_UK
dc.contributor.authorSechi, Pierfrancescoen_UK
dc.contributor.authorKille, Peteren_UK
dc.contributor.authorSpurgeon, Daviden_UK
dc.date.accessioned2017-12-06T01:34:09Z-
dc.date.available2017-12-06T01:34:09Z-
dc.date.issued2017-11-17en_UK
dc.identifier.other97en_UK
dc.identifier.urihttp://hdl.handle.net/1893/26181-
dc.description.abstractBackground  Populations of the earthworm, Lumbricus rubellus, are commonly found across highly contaminated former mine sites and are considered to have under-gone selection for mitigating metal toxicity. Comparison of adapted populations with those found on less contaminated soils can provide insights into ecological processes that demonstrate the long-term effects of soil contamination. Contemporary sequencing methods allow for portrayal of demographic inferences and highlight genetic variation indicative of selection at specific genes. Furthermore, the occurrence of L. rubellus lineages across the UK allows for inferences of mechanisms associated with drivers of speciation and local adaptation.  Results  Using RADseq, we were able to define population structure between the two lineages through the use of draft genomes for each, demonstrating an absence of admixture between lineages and that populations over extensive geographic distances form discrete populations. Between the two British lineages, we were able to provide evidence for selection near to genes associated with epigenetic and morphological functions, as well as near a gene encoding a pheromone. Earthworms inhabiting highly contaminated soils bare close genomic resemblance to those from proximal control soils. We were able to define a number of SNPs that largely segregate populations and are indicative of genes that are likely under selection for managing metal toxicity. This includes calcium and phosphate-handling mechanisms linked to lead and arsenic contaminants, respectively, while we also observed evidence for glutathione-related mechanisms, including metallothionein, across multiple populations. Population genomic end points demonstrate no consistent reduction in nucleotide diversity, or increase in inbreeding coefficient, relative to history of exposure.   Conclusions  Though we can clearly define lineage membership using genomic markers, as well as population structure between geographic localities, it is difficult to resolve markers that segregate entirely between populations in response to soil metal concentrations. This may represent a highly variable series of traits in response to the heterogenous nature of the soil environment, but ultimately demonstrates the maintenance of lineage-specific genetic variation among local populations. L. rubellus appears to provide an exemplary system for exploring drivers for speciation, with a continuum of lineages coexisting across continental Europe, while distinct lineages exist in isolation throughout the UK.en_UK
dc.language.isoenen_UK
dc.publisherBioMed Centralen_UK
dc.relationAnderson C, Cunha L, Sechi P, Kille P & Spurgeon D (2017) Genetic variation in populations of the earthworm, Lumbricus rubellus, across contaminated mine sites. BMC Genetics, 18 (1), Art. No.: 97. https://doi.org/10.1186/s12863-017-0557-8en_UK
dc.relation.urihttp://hdl.handle.net/11667/97en_UK
dc.rights© The Author(s). 2017 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectEarthwormsen_UK
dc.subjectRADseqen_UK
dc.subjectEcotoxicologyen_UK
dc.subjectPopulation genomicsen_UK
dc.subjectAdaptationen_UK
dc.subjectArsenicen_UK
dc.subjectLeaden_UK
dc.titleGenetic variation in populations of the earthworm, Lumbricus rubellus, across contaminated mine sitesen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/s12863-017-0557-8en_UK
dc.identifier.pmid29149838en_UK
dc.citation.jtitleBMC Geneticsen_UK
dc.citation.issn1471-2156en_UK
dc.citation.volume18en_UK
dc.citation.issue1en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderNatural Environment Research Councilen_UK
dc.citation.date17/11/2017en_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationCardiff Universityen_UK
dc.contributor.affiliationCardiff Universityen_UK
dc.contributor.affiliationCardiff Universityen_UK
dc.contributor.affiliationCentre for Ecology & Hydrology (CEH)en_UK
dc.identifier.isiWOS:000415631800001en_UK
dc.identifier.scopusid2-s2.0-85034017512en_UK
dc.identifier.wtid510587en_UK
dc.contributor.orcid0000-0002-2832-9313en_UK
dc.date.accepted2017-10-03en_UK
dcterms.dateAccepted2017-10-03en_UK
dc.date.filedepositdate2017-11-22en_UK
rioxxterms.apcpaiden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorAnderson, Craig|0000-0002-2832-9313en_UK
local.rioxx.authorCunha, Luis|en_UK
local.rioxx.authorSechi, Pierfrancesco|en_UK
local.rioxx.authorKille, Peter|en_UK
local.rioxx.authorSpurgeon, David|en_UK
local.rioxx.projectProject ID unknown|Natural Environment Research Council|http://dx.doi.org/10.13039/501100000270en_UK
local.rioxx.freetoreaddate2017-11-22en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2017-11-22|en_UK
local.rioxx.filenames12863-017-0557-8.pdfen_UK
local.rioxx.filecount1en_UK
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