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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: The influence of dietary fatty acid and fasting on the hepatic lipid metabolism of barramundi (Lates calcarifer)
Author(s): Araujo, Bruno C
Salini, Michael J
Glencross, Brett
Wade, Nicholas
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Keywords: Asian sea bass
gene expression
fatty acids
Issue Date: Jul-2017
Citation: Araujo BC, Salini MJ, Glencross B & Wade N (2017) The influence of dietary fatty acid and fasting on the hepatic lipid metabolism of barramundi (Lates calcarifer), Aquaculture Research, 48 (7), pp. 3879-3893.
Abstract: For many fish species, dietary fish oil (FO) has been substituted with other oils such as poultry oil (PO) without affecting growth performance. However, in barramundi, the mechanisms by which fatty acid metabolism is regulated are poorly understood, and the effects of FO substitution are unknown. This study defined changes in the expression of genes controlling the metabolism of fatty acids in barramundi over a 24-h time period after a single meal. From one to 12h after a single feeding event, the expression of fatty acid synthesis genes in the liver was upregulated, while genes involved in the β-oxidation showed minimal alteration. However, the expression of β-oxidation genes was significantly correlated with the expression of genes regulating fatty acid synthesis. In a second experiment, the changes in liver fatty acid composition and gene expression were defined after FO was substituted with PO. Liver fatty acid profile reflected the diet composition, with some subtle exceptions supporting the enrichment of certain long-chain polyunsaturated fatty acids in the liver. The fish from all experimental groups preferentially retained more docosahexaenoic acid than eicosapentaenoic acid in the liver, suggesting a bioconversion of this fatty acid to intermediate fatty acids. Replacement of FO with PO significantly regulated genes controlling both fatty acid synthesis and catabolism pathways, potentially related to a higher percentage of monounsaturated fatty acids, in the livers of fish fed these diets. The results demonstrated that diet composition significantly altered the lipid metabolism in barramundi and that there was a balance between direct dietary effects and endogenous synthetic capacity.
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Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Araújo, B., Salini, M., Glencross, B. and Wade, N. (2017), The influence of dietary fatty acid and fasting on the hepatic lipid metabolism of barramundi (Lates calcarifer). Aquac Res, 48: 3879–3893, which has been published in final form at This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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