|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Antimicrobial susceptibility of Flavobacterium psychrophilum isolates from the United Kingdom|
|Author(s):||Ngo, Thao P H|
Thompson, Kim D
Verner-Jeffreys, David W
epidemiological cut-off values
rainbow trout fry syndrome
|Citation:||Ngo TPH, Smith P, Bartie K, Thompson KD, Verner-Jeffreys DW, Hoare R & Adams A (2018) Antimicrobial susceptibility of Flavobacterium psychrophilum isolates from the United Kingdom, Journal of Fish Diseases, 41 (2), pp. 309-320.|
|Abstract:||Routine application of antimicrobials is the current treatment of choice for rainbow trout fry syndrome (RTFS) or bacterial coldwater disease (BCWD) caused by Flavobacterium psychrophilum. In this study, the antimicrobial susceptibilities of 133 F. psychrophilum isolates, 118 of which were from the UK, were evaluated by broth microdilution and disc diffusion methods following VET04-A2 and VET03-A guidelines of Clinical and Laboratory Standards Institute (CLSI), respectively. Isolates were categorised as wild type (fully susceptible, WT) or non-wild type (NWT) using normalised resistance interpretation (NRI) determined cut-off values (COWT). Broth microdilution testing showed that only 12% of UK isolates were WT to oxolinic acid (MIC COWT ≤0.25 mg L-1) and 42% were WT for oxytetracycline (MIC COWT ≤0.25 mg L-1). In contrast, all the isolates tested were WT (MIC COWT ≤2 mg L-1) for florfenicol, the main antimicrobial for RTFS control in the UK. Disc diffusion-based COWT values were ≥51 mm for 10 µg amoxicillin, ≥44 mm for 30 µg florfenicol, ≥30 mm for 2 µg oxolinic acid and ≥51 mm for 30 µg oxytetracycline. There was a high categorical agreement between the classifications of the isolates by two testing methods for florfenicol (100%), oxytetracycline (93%), and oxolinic acid (99%).|
|Rights:||This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Ngo TPH, Smith P, Bartie KL, et al. Antimicrobial susceptibility of Flavobacterium psychrophilum isolates from the United Kingdom. Journal of Fish Diseases 2018;41:309–320, which has been published in final form at https://doi.org/10.1111/jfd.12730. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.|
|Manuscript-Fp_AMR_accepted.pdf||687.59 kB||Adobe PDF||Under Embargo until 2018-10-25 Request a copy|
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