Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25706
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dc.contributor.authorXia, Liqinen_UK
dc.contributor.authorLiang, Haiyingen_UK
dc.contributor.authorXu, Liangen_UK
dc.contributor.authorChen, Jianlinen_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorZhang, Honglianen_UK
dc.contributor.authorYu, Lishanen_UK
dc.date.accessioned2018-01-10T23:39:44Z-
dc.date.available2018-01-10T23:39:44Z-
dc.date.issued2017-09en_UK
dc.identifier.otherfnx143en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25706-
dc.description.abstractFish nocardiosis is a chronic systemic granulomatous disease, andNocardia seriolaeis the main pathogen that causes this disease. But the pathogenesis and virulence factors ofN. seriolaeare not fully understood. A phospholipase C (PLC), which was likely to be a secreted protein targeting host cell mitochondria, was found by the bioinformatics analysis on the whole genome sequence ofN. seriolae. In order to determine the subcellular localization and study the preliminary function of PLC fromN. seriolae(NsPLC), the gene cloning, secreted protein identification, subcellular localization in host cells and apoptosis detection of NsPLC were carried out in this study. The results showed that NsPLC was a secreted protein by mass spectrometry analysis of extracellular products fromN. seriolae. Subcellular localization of NsPLC-GFP fusion protein in FHM cells revealed that the green fluorescence exhibited a punctate distribution near the nucleus and did not co-localize with mitochondria. In addition, apoptosis assay suggested that apoptosis was induced in FHM cells by the overexpression of NsPLC. This study may lay the foundation for further study on the function of NsPLC and promote the understanding of the virulence factors and pathogenic mechanism ofN. seriolae.en_UK
dc.language.isoenen_UK
dc.publisherOxford University Pressen_UK
dc.relationXia L, Liang H, Xu L, Chen J, Bekaert M, Zhang H & Yu L (2017) Subcellular localization and function study of a secreted phospholipase C from Nocardia seriolae. FEMS Microbiology Letters, 364 (17), Art. No.: fnx143. https://doi.org/10.1093/femsle/fnx143en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectNocardia seriolaeen_UK
dc.subjectphospholipase Cen_UK
dc.subjectsubcellular localizationen_UK
dc.subjectsecreted proteinen_UK
dc.subjectcell apoptosisen_UK
dc.subjectoverexpressionen_UK
dc.titleSubcellular localization and function study of a secreted phospholipase C from Nocardia seriolaeen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[fnx143.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1093/femsle/fnx143en_UK
dc.identifier.pmid28911184en_UK
dc.citation.jtitleFEMS Microbiology Lettersen_UK
dc.citation.issn1574-6968en_UK
dc.citation.issn0378-1097en_UK
dc.citation.volume364en_UK
dc.citation.issue17en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailmichael.bekaert@stir.ac.uken_UK
dc.citation.date07/07/2017en_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.identifier.isiWOS:000412848800002en_UK
dc.identifier.scopusid2-s2.0-85037693124en_UK
dc.identifier.wtid522139en_UK
dc.contributor.orcid0000-0002-1206-7654en_UK
dc.date.accepted2017-06-30en_UK
dcterms.dateAccepted2017-06-30en_UK
dc.date.filedepositdate2017-08-07en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorXia, Liqin|en_UK
local.rioxx.authorLiang, Haiying|en_UK
local.rioxx.authorXu, Liang|en_UK
local.rioxx.authorChen, Jianlin|en_UK
local.rioxx.authorBekaert, Michaël|0000-0002-1206-7654en_UK
local.rioxx.authorZhang, Honglian|en_UK
local.rioxx.authorYu, Lishan|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2018-07-08en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2018-07-07en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2018-07-08|en_UK
local.rioxx.filenamefnx143.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0378-1097en_UK
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