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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Gene-centromere mapping in meiotic gynogenetic European seabass
Author(s): Oral, Münevver
Colléter, Julie
Bekaert, Michaël
Taggart, John B
Palaiokostas, Christos
McAndrew, Brendan J
Vandeputte, Marc
Chatain, Béatrice
Kuhl, Heiner
Reinhardt, Richard
Peruzzi, Stefano
Penman, David J
Keywords: Dicentrarchus labrax
Meiotic gynogenesis
Isogenic lines
ddRAD seq
Genetic map
Gene-Centromere map
Issue Date: 7-Jun-2017
Citation: Oral M, Colleter J, Bekaert M, Taggart J, Palaiokostas C, McAndrew B, Vandeputte M, Chatain B, Kuhl H, Reinhardt R, Peruzzi S & Penman DJ (2017) Gene-centromere mapping in meiotic gynogenetic European seabass, BMC Genomics, 18 (1), Art. No.: 449.
Project Number 262336
Abstract: Background  Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families.  Results  From a single meiotic gynogenetic family consisting of 79 progeny, 42 million sequencing reads (Illumina, trimmed to 148 bases) resolved 6866 unique RAD-tags. The 340 male-informative SNP markers that were identified confirmed the lack of paternal contribution. A gene-centromere map was constructed based on 804 female-informative SNPs in 24 linkage groups (2n=48) with a total length of 1251.02cM (initial LG assignment was based on the seabass genome assembly, dicLab v1). Chromosome arm structure could be clearly discerned from the pattern of heterozygosity in each linkage group in 18 out of 24 LGs: the other six showed anomalies that appeared to be related to issues in the genome assembly.  Conclusion  Genome-wide screening enabled substantive verification of the production of the gynogenetic family used in this study. The large number of telomeric and subtelomeric markers with high heterozygosity values in the meiotic gynogenetic family indicate that such markers could be used to clearly distinguish between meiotic and mitotic gynogenetics.
DOI Link: 10.1186/s12864-017-3826-z
Rights: © The Author(s). 2017 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

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