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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Ultrastructural analysis of sequential cyprinid herpesvirus 3 morphogenesis in vitro
Author(s): Monaghan, Sean
Bergmann, Sven
Thompson, Kim D
Brown, Linton
Herath, Tharangani
del-Pozo, Jorge
Adams, Alexandra
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Keywords: cyprinid herpesvirus 3
koi herpesvirus
sequential morphogenesis
transmission electronmicroscopy
Issue Date: 31-Aug-2017
Citation: Monaghan S, Bergmann S, Thompson KD, Brown L, Herath T, del-Pozo J & Adams A (2017) Ultrastructural analysis of sequential cyprinid herpesvirus 3 morphogenesis in vitro. Journal of Fish Diseases, 40 (8), pp. 1041-1054.
Abstract: Cyprinid herpesvirus 3 (CyHV-3) is an alloherpesvirus, and it is the aetiological agent of koi herpesvirus disease. Although the complex morphogenic stages of the replication cycle of CyHV-3 were shown to resemble that of other members of the Herpesvirales, detailed analysis of the sequence and timing of these events was not definitively determined. This study describes these features through a time course using cyprinid cell cultures (KF-1 and CCB) infected with CyHV-3 (KHV isolate, H361) and analysed by transmission electron microscopy. Rapid viral entry was noted, with high levels of intracellular virus within 1–4 h post-infection (hpi). Intranuclear capsid assembly, paracrystalline array formation and primary envelopment of capsids occurred within 4 hpi. Between 1 and 3 days post-infection (dpi), intracytoplasmic secondary envelopment occurred, as well as budding of infectious virions at the plasma membrane. At 5–7 dpi, the cytoplasm contained cytopathic vacuoles, enveloped virions within vesicles, and abundant non-enveloped capsids; also there was frequent nuclear deformation. Several morphological features are suggestive of inefficient viral assembly, with production of non-infectious particles, particularly in KF-1 cells. The timing of this alloherpesvirus morphogenesis is similar to other members of the Herpesvirales, but there may be possible implications of using different cell lines for CyHV-3 propagation.
DOI Link: 10.1111/jfd.12580
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Monaghan, S. J., Bergmann, S. M., Thompson, K. D., Brown, L., Herath, T., del-Pozo, J. and Adams, A. (2017), Ultrastructural analysis of sequential cyprinid herpesvirus 3 morphogenesis in vitro. J Fish Dis, 40: 1041–1054, which has been published in final form at This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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