Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25108
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dc.contributor.authorByrne, C Jen_UK
dc.contributor.authorFair, Seanen_UK
dc.contributor.authorEnglish, A Men_UK
dc.contributor.authorHolden, Shauna Aen_UK
dc.contributor.authorDick, James Ren_UK
dc.contributor.authorLonergan, Patricken_UK
dc.contributor.authorKenny, David Aen_UK
dc.date.accessioned2017-04-21T02:21:58Z-
dc.date.available2017-04-21T02:21:58Z-
dc.date.issued2017-03-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25108-
dc.description.abstractThe aim of this study was to examine the effects of dietary supplementation with rumen protected n-6 or n-3 polyunsaturated fatty acids (PUFA) on the quantity and quality of semen from young post-pubertal dairy bulls. Pubertal Holstein-Friesian (n=43) and Jersey (n=7) bulls with a mean±s.e.m. age and bodyweight of 420.1±5.86 days and 382±8.94kg, respectively, were blocked on breed, weight, age and semen quality (based on the outcomes of two pre-trial ejaculates) and randomly assigned to one of three treatments: (i) a non-supplemented control (CTL, n=15), (ii) rumen-protected safflower (SO, n=15), (iii) rumen-protected n-3 PUFA-enriched fish oil (FO, n=20). Bulls were fed their respective diets,ad libitumfor 12 weeks; individual intakes were recorded using an electronic feeding system for the initial 6 weeks of the feeding period. Semen was collected via electro-ejaculation at weeks−2,−1, 0, 7, 10, 11 and 12 relative to the beginning of the trial period (week 0). On collection, semen volume, sperm concentration and progressive linear motility (PLM) were assessed. On weeks−2,−1, 0, 10, 11, 12, semen was packaged into 0.25mL straws and frozen using a programmable freezer. On weeks−1, 7 and 11; a sub-sample of semen was separated into sperm and seminal plasma, by centrifugation and stored at – 20°C until analysis of lipid composition. Semen from 10 bulls per treatment were used for post-thaw analysis at weeks 10, 11 and 12 (3 straws per ejaculate). Sperm motility was analysed by computer assisted semen analysis (CASA). In addition, membrane fluidity, acrosome reaction and oxidative stress were assessed using flow cytometry. Sperm from bulls fed SO had a 1.2 fold higher total n-6 PUFA content at week 11 compared to week−1 (P<0.01) while bulls fed FO had a 1.3 fold higher total n-3 PUFA content, in sperm by week 11 (P<0.01). There was no effect of diet on semen volume, concentration or PLM of sperm when assessed either immediately following collection or post-thawing. Membrane fluidity and oxidative stress of sperm were also not affected by diet. The percentage of sperm with intact-acrosomes was lower in CTL bulls compared to those fed SO (P<0.01). In conclusion, while the lipid composition of semen was altered following dietary supplementation with either n-6 or n-3 based PUFA, this did not lead to measurable improvements in the quantity or quality of semen produced by young post-pubertal dairy bulls.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationByrne CJ, Fair S, English AM, Holden SA, Dick JR, Lonergan P & Kenny DA (2017) Dietary polyunsaturated fatty acid supplementation of young post-pubertal dairy bulls alters the fatty acid composition of seminal plasma and spermatozoa but has no effect on semen volume or sperm quality. Theriogenology, 90, pp. 289-300. https://doi.org/10.1016/j.theriogenology.2016.12.014en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Byrne CJ, Fair S, English AM, Holden SA, Dick JR, Lonergan P & Kenny DA (2017) Dietary polyunsaturated fatty acid supplementation of young post-pubertal dairy bulls alters the fatty acid composition of seminal plasma and spermatozoa but has no effect on semen volume or sperm quality, Theriogenology, 90, pp. 289-300. DOI: 10.1016/j.theriogenology.2016.12.014 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectFertilityen_UK
dc.subjectSemenen_UK
dc.subjectPUFAen_UK
dc.subjectLipid compositionen_UK
dc.subjectFish oilen_UK
dc.subjectSaffloweren_UK
dc.titleDietary polyunsaturated fatty acid supplementation of young post-pubertal dairy bulls alters the fatty acid composition of seminal plasma and spermatozoa but has no effect on semen volume or sperm qualityen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2017-12-19en_UK
dc.rights.embargoreason[Byrne_et_al_2016_7th_December_2016_revised (002).pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.theriogenology.2016.12.014en_UK
dc.identifier.pmid28166982en_UK
dc.citation.jtitleTheriogenologyen_UK
dc.citation.issn0093-691Xen_UK
dc.citation.volume90en_UK
dc.citation.spage289en_UK
dc.citation.epage300en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailj.r.dick@stir.ac.uken_UK
dc.citation.date18/12/2016en_UK
dc.contributor.affiliationUniversity College Dublin (UCD)en_UK
dc.contributor.affiliationUniversity of Limerick, Irelanden_UK
dc.contributor.affiliationUniversity of Limerick, Irelanden_UK
dc.contributor.affiliationUniversity of Limerick, Irelanden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity College Dublin (UCD)en_UK
dc.contributor.affiliationUniversity College Dublin (UCD)en_UK
dc.identifier.isiWOS:000394195400040en_UK
dc.identifier.scopusid2-s2.0-85008707909en_UK
dc.identifier.wtid538198en_UK
dc.date.accepted2016-12-12en_UK
dcterms.dateAccepted2016-12-12en_UK
dc.date.filedepositdate2017-03-07en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorByrne, C J|en_UK
local.rioxx.authorFair, Sean|en_UK
local.rioxx.authorEnglish, A M|en_UK
local.rioxx.authorHolden, Shauna A|en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.authorLonergan, Patrick|en_UK
local.rioxx.authorKenny, David A|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2017-12-19en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2017-12-18en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2017-12-19|en_UK
local.rioxx.filenameByrne_et_al_2016_7th_December_2016_revised (002).pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0093-691Xen_UK
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