Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24991
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dc.contributor.authorSubramanian, Mahendranen_UK
dc.contributor.authorTyler, Aimee-Jayneen_UK
dc.contributor.authorLuther, Eva Mariaen_UK
dc.contributor.authorDi Daniel, Elenaen_UK
dc.contributor.authorLim, Jensonen_UK
dc.contributor.authorDobson, Johnen_UK
dc.date.accessioned2017-08-25T22:19:34Z-
dc.date.available2017-08-25T22:19:34Z-
dc.date.issued2017-01-29en_UK
dc.identifier.other28en_UK
dc.identifier.urihttp://hdl.handle.net/1893/24991-
dc.description.abstractTo develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types.en_UK
dc.language.isoenen_UK
dc.publisherMDPIen_UK
dc.relationSubramanian M, Tyler A, Luther EM, Di Daniel E, Lim J & Dobson J (2017) Oscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies. Nanomaterials, 7 (2), Art. No.: 28. http://www.mdpi.com/2079-4991/7/2/28; https://doi.org/10.3390/nano7020028en_UK
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectmagnetic nanoparticlesen_UK
dc.subjecttransfectionen_UK
dc.subjectSH-SY5Yen_UK
dc.subjecthippocampal neuronsen_UK
dc.subjectcortical neuronsen_UK
dc.subjectnon-viral gene deliveryen_UK
dc.subjectnanomagnetic gene transfectionen_UK
dc.subjectbiomagneticsen_UK
dc.subjectgradient magnetic fielden_UK
dc.subjectpermanent magnetsen_UK
dc.titleOscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studiesen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.3390/nano7020028en_UK
dc.identifier.pmid28336862en_UK
dc.citation.jtitleNanomaterialsen_UK
dc.citation.issn2079-4991en_UK
dc.citation.volume7en_UK
dc.citation.issue2en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.identifier.urlhttp://www.mdpi.com/2079-4991/7/2/28en_UK
dc.author.emailjenson.lim@stir.ac.uken_UK
dc.citation.date29/01/2017en_UK
dc.contributor.affiliationnanoTherics Ltden_UK
dc.contributor.affiliationnanoTherics Ltden_UK
dc.contributor.affiliationnanoTherics Ltden_UK
dc.contributor.affiliationTakedaen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationUniversity of Floridaen_UK
dc.identifier.isiWOS:000400761600006en_UK
dc.identifier.scopusid2-s2.0-85011928388en_UK
dc.identifier.wtid536817en_UK
dc.contributor.orcid0000-0001-7417-356Xen_UK
dc.date.accepted2017-01-23en_UK
dcterms.dateAccepted2017-01-23en_UK
dc.date.filedepositdate2017-02-22en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorSubramanian, Mahendran|en_UK
local.rioxx.authorTyler, Aimee-Jayne|en_UK
local.rioxx.authorLuther, Eva Maria|en_UK
local.rioxx.authorDi Daniel, Elena|en_UK
local.rioxx.authorLim, Jenson|0000-0001-7417-356Xen_UK
local.rioxx.authorDobson, John|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2017-02-22en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2017-02-22|en_UK
local.rioxx.filenamenanomaterials-07-00028.pdfen_UK
local.rioxx.filecount1en_UK
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