Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24981
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dc.contributor.authorNgo, Thao P Hen_UK
dc.contributor.authorBartie, Kerryen_UK
dc.contributor.authorThompson, Kim Den_UK
dc.contributor.authorVerner-Jeffreys, David Wen_UK
dc.contributor.authorHoare, Rowenaen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.date.accessioned2017-04-21T02:59:45Z-
dc.date.available2017-04-21T02:59:45Z-
dc.date.issued2017-03en_UK
dc.identifier.urihttp://hdl.handle.net/1893/24981-
dc.description.abstractFlavobacterium psychrophilum is one of the most important bacterial pathogens affecting cultured rainbow trout (Oncorhynchus mykiss) and is increasingly causing problems in Atlantic salmon (Salmo salar L.) hatcheries. Little is known about the heterogeneity of F. psychrophilum isolates on UK salmonid farms. A total of 315 F. psychrophilum isolates, 293 of which were collected from 27 sites within the UK, were characterised using four genotyping methods and a serotyping scheme. A high strain diversity was identified among the isolates with 54 pulsotypes, ten (GTG)5-PCR types, two 16S rRNA allele lineages, seven plasmid profiles and three serotypes. Seven PFGE groups and 27 singletons were formed at a band similarity of 80%. PFGE group P (n=75) was found to be numerically predominant in eight sites within the UK. Two major PFGE clusters and 13 outliers were found at the band similarity of 40%. The predominant profile observed within the F. psychrophilum isolates examined was PFGE cluster II − (GTG)5-PCR type r1–16S rRNA lineage II − serotype Th (70/156 isolates examined, 45%). Co-existence of genetically and serologically heterogeneous isolates within each farm was detected, confounding the ability to control RTFS outbreaks. The occurrence over time (up to 11 years) of F. psychrophilum pulsotypes in three representative sites (Scot I, Scot III and Scot V) within Scotland was examined, potentially providing important epidemiological data for farm management and the development of site-specific vaccines.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationNgo TPH, Bartie K, Thompson KD, Verner-Jeffreys DW, Hoare R & Adams A (2017) Genetic and serological diversity of Flavobacterium psychrophilum isolates from salmonids in United Kingdom. Veterinary Microbiology, 201, pp. 216-224. https://doi.org/10.1016/j.vetmic.2017.01.032en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Ngo TPH, Bartie K, Thompson KD, Verner-Jeffreys DW, Hoare R & Adams A (2017) Genetic and serological diversity of Flavobacterium psychrophilum isolates from salmonids in United Kingdom, Veterinary Microbiology, 201, pp. 216-224. DOI: 10.1016/j.vetmic.2017.01.032 © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectRainbow trout fry syndromeen_UK
dc.subjectBacterial cold water diseaseen_UK
dc.subjectFlavobacterium psychrophilumen_UK
dc.subjectGenotypingen_UK
dc.subjectSerotypingen_UK
dc.subjectPlasmidsen_UK
dc.titleGenetic and serological diversity of Flavobacterium psychrophilum isolates from salmonids in United Kingdomen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2018-02-01en_UK
dc.rights.embargoreason[Manuscript (revised).pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.vetmic.2017.01.032en_UK
dc.identifier.pmid28284613en_UK
dc.citation.jtitleVeterinary Microbiologyen_UK
dc.citation.issn0378-1135en_UK
dc.citation.volume201en_UK
dc.citation.spage216en_UK
dc.citation.epage224en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderEuropean Commissionen_UK
dc.author.emailrowena.hoare1@stir.ac.uken_UK
dc.citation.date31/01/2017en_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationCEFAS - Centre for Environment, Fisheries and Aquaculture Scienceen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000397689800031en_UK
dc.identifier.scopusid2-s2.0-85011634501en_UK
dc.identifier.wtid536225en_UK
dc.contributor.orcid0000-0003-3037-5295en_UK
dc.contributor.orcid0000-0002-9298-4275en_UK
dc.date.accepted2017-01-27en_UK
dcterms.dateAccepted2017-01-27en_UK
dc.date.filedepositdate2017-02-17en_UK
dc.relation.funderprojectTargeted disease prophylaxis in European fish farmingen_UK
dc.relation.funderref311993en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorNgo, Thao P H|en_UK
local.rioxx.authorBartie, Kerry|0000-0003-3037-5295en_UK
local.rioxx.authorThompson, Kim D|en_UK
local.rioxx.authorVerner-Jeffreys, David W|en_UK
local.rioxx.authorHoare, Rowena|0000-0002-9298-4275en_UK
local.rioxx.authorAdams, Alexandra|en_UK
local.rioxx.project311993|European Commission (Horizon 2020)|en_UK
local.rioxx.freetoreaddate2018-02-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2018-01-31en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2018-02-01|en_UK
local.rioxx.filenameManuscript (revised).pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0378-1135en_UK
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