Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24959
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dc.contributor.authorFaye, Martinen_UK
dc.contributor.authorDacheux, Laurenten_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorDiop, Sylvie Audreyen_UK
dc.contributor.authorLoucoubar, Cheikhen_UK
dc.contributor.authorBourhy, Herveen_UK
dc.contributor.authorSall, Amadou Aen_UK
dc.contributor.authorFaye, Ousmaneen_UK
dc.date.accessioned2017-08-26T04:19:42Z-
dc.date.available2017-08-26T04:19:42Z-
dc.date.issued2017-05en_UK
dc.identifier.urihttp://hdl.handle.net/1893/24959-
dc.description.abstractRabies virus (RABV) remains one of the most important global zoonotic pathogens. RABV causes rabies, an acute encephalomyelitis associated with a high rate of mortality in humans and animals and affecting different parts of the world, particularly in Asia and Africa. Confirmation of rabies diagnosis relies on laboratory diagnosis, in which molecular techniques such as detection of viral RNA by reverse transcription polymerase chain reaction (RT-PCR) are increasingly being used.  In this study, two real-time quantitative RT-PCR assays were developed for large-spectrum detection of RABV, with a focus on African isolates. The primer and probe sets were targeted highly conserved regions of the nucleoprotein (N) and polymerase (L) genes.  The results indicated the absence of non-specific amplification and cross-reaction with a range of other viruses belonging to the same taxonomic family, i.e Rhabdoviridae, as well as negative brain tissues from various host species. Analytical sensitivity ranged between 100 to 10 standard RNA copies detected per reaction for N-gene and L-gene assays, respectively. Effective detection and high sensitivity of these assays on African isolates showed that they can be successfully applied in general research and used in diagnostic process and epizootic surveillance in Africa using a double-check strategy.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationFaye M, Dacheux L, Weidmann M, Diop SA, Loucoubar C, Bourhy H, Sall AA & Faye O (2017) Development and validation of sensitive real-time RT-PCR assay for broad detection of rabies virus. Journal of Virological Methods, 243, pp. 120-130. https://doi.org/10.1016/j.jviromet.2016.12.019en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Faye M, Dacheux L, Weidmann M, Diop SA, Loucoubar C, Bourhy H, Sall AA & Faye O (2017) Development and validation of sensitive real-time RT-PCR assay for broad detection of rabies virus, Journal of Virological Methods, 243, pp. 120-130. DOI: 10.1016/j.jviromet.2016.12.019 © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectRabies virus (RABV)en_UK
dc.subjectReal-time RT-qPCR assaysen_UK
dc.subjectmolecular techniquesen_UK
dc.subjectbroad detectionen_UK
dc.subjectAfricaen_UK
dc.titleDevelopment and validation of sensitive real-time RT-PCR assay for broad detection of rabies virusen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2018-02-05en_UK
dc.rights.embargoreason[Faye_etal_JournalofVirologicalMethods_2017.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.jviromet.2016.12.019en_UK
dc.identifier.pmid28174073en_UK
dc.citation.jtitleJournal of Virological Methodsen_UK
dc.citation.issn0166-0934en_UK
dc.citation.volume243en_UK
dc.citation.spage120en_UK
dc.citation.epage130en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.citation.date04/02/2017en_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteuren_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity Hospital Center of Fannen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteuren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.identifier.isiWOS:000398643900021en_UK
dc.identifier.scopusid2-s2.0-85012972071en_UK
dc.identifier.wtid536057en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2016-12-27en_UK
dcterms.dateAccepted2016-12-27en_UK
dc.date.filedepositdate2017-02-10en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorFaye, Martin|en_UK
local.rioxx.authorDacheux, Laurent|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorDiop, Sylvie Audrey|en_UK
local.rioxx.authorLoucoubar, Cheikh|en_UK
local.rioxx.authorBourhy, Herve|en_UK
local.rioxx.authorSall, Amadou A|en_UK
local.rioxx.authorFaye, Ousmane|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2018-02-05en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2018-02-04en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2018-02-05|en_UK
local.rioxx.filenameFaye_etal_JournalofVirologicalMethods_2017.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0166-0934en_UK
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