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dc.contributor.authorHeumann, Janen_UK
dc.contributor.authorCarmichael, Stephen Nen_UK
dc.contributor.authorBron, Jamesen_UK
dc.contributor.authorTildesley, Andrewen_UK
dc.contributor.authorSturm, Arminen_UK
dc.description.abstractThe salmon louse, Lepeophtheirus salmonis, is a crustacean ectoparasite of salmonid fish. At present, sea louse control on salmon farms relies heavily upon chemical treatments. Drug efflux transport, mediated by ABC transporters such as P-glycoprotein (Pgp), represents a major mechanism for drug resistance in parasites. We report here the molecular cloning of a new Pgp from the salmon louse, called SL-PGY1. A partial Pgp sequence was obtained by searching sea louse ESTs, and extended by rapid amplification of cDNA ends (RACE). The open reading frame of SL-PGY1 encodes a protein of 1438 amino acids that possesses typical structural traits of P-glycoproteins, and shows a high degree of sequence homology to invertebrate and vertebrate P-glycoproteins. In the absence of drug exposure, SL-PGY1 mRNA expression levels did not differ between a drug-susceptible strain of L. salmonis and a strain showing a ~ 7-fold decrease in sensitivity against emamectin benzoate, the active component of the in-feed sea louse treatment SLICE® (Merck Animal Health). Aqueous exposure of the hyposensitive salmon louse strain to emamectin benzoate (24 h, 410 μg/L) provoked a 2.9-fold upregulation of SL-PGY1. Adult male lice of both strains showed a greater abundance of SL-PGY1 mRNA than adult females.en_UK
dc.relationHeumann J, Carmichael SN, Bron J, Tildesley A & Sturm A (2012) Molecular cloning and characterisation of a novel P-glycoprotein in the salmon louse Lepeophtheirus salmonis. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 155 (2), pp. 198-205.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectABC transporteren_UK
dc.subjectSea louseen_UK
dc.subjectEmamectin benzoateen_UK
dc.subjectSLICE (R)en_UK
dc.subjectDrug resistanceen_UK
dc.titleMolecular cloning and characterisation of a novel P-glycoprotein in the salmon louse Lepeophtheirus salmonisen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[CBPC 2011.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part C: Toxicology and Pharmacologyen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorHeumann, Jan|en_UK
local.rioxx.authorCarmichael, Stephen N|en_UK
local.rioxx.authorBron, James|0000-0003-3544-0519en_UK
local.rioxx.authorTildesley, Andrew|en_UK
local.rioxx.authorSturm, Armin|0000-0003-2632-1999en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameCBPC 2011.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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