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dc.contributor.authorShalaby, Mohamed Aen_UK
dc.contributor.authorEl-Deeb, Aymanen_UK
dc.contributor.authorEl-Tholoth, Mohameden_UK
dc.contributor.authorHoffmann, Donataen_UK
dc.contributor.authorCzerny, Claus-Peteren_UK
dc.contributor.authorHufert, Frank Ten_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorEl Wahed, Ahmed Abden_UK
dc.description.abstractBackground  Lumpy skin disease virus (LSDV) is aCapripoxvirusinfecting cattle and Buffalos. Lumpy skin disease (LSD) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). Early detection of the virus is crucial to start appropriate outbreak control measures. Veterinarians rely on the presence of the characteristic clinical signs of LSD. Laboratory diagnostics including virus isolation, sequencing and real-time polymerase chain reaction (PCR) are performed at well-equipped laboratories. In this study, a portable, simple, and rapid recombinase polymerase amplification (RPA) assay for the detection of LSDV-genome for the use on farms was developed.  Results  The LSDV RPA assay was performed at 42 °C and detected down to 179 DNA copies/reaction in a maximum of 15 min. Unspecific amplification was observed with neither LSDV-negative samples (n= 12) nor nucleic acid preparations from orf virus, bovine papular stomatitis virus, cowpoxvirus, Peste des petits ruminants and Blue tongue virus (serotypes 1, 6 and 8). The clinical sensitivity of the LSDV RPA assay matched 100 % (n= 22) to real-time PCR results. In addition, the LSDV RPA assay detected sheep and goat poxviruses.  Conclusion  The LSDV RPA assay is a rapid and sensitive test that could be implemented in field or at quarantine stations for the identification of LSDV infected case.en_UK
dc.publisherBioMed Centralen_UK
dc.relationShalaby MA, El-Deeb A, El-Tholoth M, Hoffmann D, Czerny C, Hufert FT, Weidmann M & El Wahed AA (2016) Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus. BMC Veterinary Research, 12 (1), Art. No.: 244.
dc.rights© The Author(s). 2016 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.en_UK
dc.subjectLumpy skin disease virusen_UK
dc.subjectRecombinase polymerase amplification assayen_UK
dc.subjectPoint of need testen_UK
dc.titleRecombinase polymerase amplification assay for rapid detection of lumpy skin disease virusen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleBMC Veterinary Researchen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationCairo Universityen_UK
dc.contributor.affiliationCairo Universityen_UK
dc.contributor.affiliationMansoura Universityen_UK
dc.contributor.affiliationFriedrich-Loeffler Instituten_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationBrandenburg Medical School Theodor-Fontaneen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorShalaby, Mohamed A|en_UK
local.rioxx.authorEl-Deeb, Ayman|en_UK
local.rioxx.authorEl-Tholoth, Mohamed|en_UK
local.rioxx.authorHoffmann, Donata|en_UK
local.rioxx.authorCzerny, Claus-Peter|en_UK
local.rioxx.authorHufert, Frank T|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorEl Wahed, Ahmed Abd|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameShalaby_etal_BMCVR_2017 (1).pdfen_UK
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