Please use this identifier to cite or link to this item:
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Remarkable resistance to UVB of the marine bacterium Photobacterium angsutum explained by an unexpected role of photolyase
Author(s): Matallana-Surget, Sabine
Douki, Thierry
Cavicchioli, Rick
Joux, Fabien
Contact Email:
Issue Date: Sep-2009
Citation: Matallana-Surget S, Douki T, Cavicchioli R & Joux F (2009) Remarkable resistance to UVB of the marine bacterium Photobacterium angsutum explained by an unexpected role of photolyase, Photochemical and Photobiological Sciences, 8 (9), pp. 1313-1320.
Abstract: DNA damage and cell survival was assessed in the marine bacteria, Photobacterium angustum (GC% = 39.6) and Sphingopyxis alaskensis (GC% = 65.5) following UVB irradiation and recovery in the presence or absence of visible light. The extent of bipyrimidine photoproduct formation was analyzed by HPLC-MS/MS. S. alaskensis was chosen as a reference species since it was previously shown to be photoresistant. Interestingly, P. angustum exhibited an even higher level of survival to UVB irradiation than S. alaskensis. This higher photoresistance was associated with a decrease in the rate of formation of cyclobutane pyrimidine dimers (CPDs) at high UVB doses. Despite different distributions in UVB-induced lesions, the survival difference between the two marine bacteria could not be accounted for by qualitative differences in either photoreactivation or the rate of nucleotide excision repair of the photoproducts arising from the different bipyrimidine doublets (TT, CT, TC and CC). Dark repair was found to be much more efficient for P. angustum than S. alaskensis but the corresponding rate of photoproduct removal was lower than that observed at high UVB doses. We propose that the increased resistance of P. angustum under high UVB doses results from a UVB-induction of CPD photolyase(s) that may directly repair DNA damage and/or act indirectly by enhancing the rate of nucleotide excision repair. © 2009 The Royal Society of Chemistry and Owner Societies.
DOI Link:
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.

Files in This Item:
File Description SizeFormat 
remarkable resistance of photobacterium.pdf395.82 kBAdobe PDFUnder Permanent Embargo    Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.