Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Triacylglycerol-, wax ester- and sterol ester-hydrolases in midgut of Atlantic salmon (Salmo salar)
Author(s): Bogevik, Andre S
Tocher, Douglas R
Waagbo, Rune
Olsen, Rolf E
Contact Email:
Keywords: bile salt-dependent lipase
triacylglycerol lipase
wax ester hydrolase
wax ester
Issue Date: Feb-2008
Date Deposited: 14-Oct-2010
Citation: Bogevik AS, Tocher DR, Waagbo R & Olsen RE (2008) Triacylglycerol-, wax ester- and sterol ester-hydrolases in midgut of Atlantic salmon (Salmo salar). Aquaculture Nutrition, 14 (1), pp. 93-98.
Abstract: Bile salt-dependent lipase (BSDL) is assumed to be the predominant lipid hydrolase in fish digestive tracts where it hydrolyses dietary triacylglycerols (TAG), sterol esters (SE) and wax esters (WE). BSDL is known to hydrolyse TAG at much faster rates than SE and WE in both fish and mammals. An assay for BSDL has previously been developed for rainbow trout (Oncorhynchus mykiss). However, this setup may not be valid in other fish species. Accordingly, the present study aimed at optimizing previous assays in rainbow trout for use on intestinal luminal contents of Atlantic salmon (Salmo salar L.). Crude intestinal extracts from midgut were desalted before the assay and concentrated bile salts supplemented. In general, the rank order for the degree of hydrolysis in Atlantic salmon was TAG > WE > SE. The optimal assay conditions were determined as being 100 lg protein, 125 lM lipid substrate and 20 mM bile salt (taurocholate) during the 4 h of incubation. Atlantic salmon and rainbow trout of 1500 g showed similar lipolytic activity, while salmon smolts of 300 g showed a significantly lower activity. Furthermore, the inhibition of intestinal lipase activities, especially triacylglycerol hydrolase and sterol ester hydrolase, observed in trout intestinal extracts at bile salt concentrations around 10 mM, was not observed in salmon. This could indicate that the activities in these two salmonids may display different enzyme biochemistry.
DOI Link: 10.1111/j.1365-2095.2007.00510.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.
Licence URL(s):

Files in This Item:
File Description SizeFormat 
Lipid hydrolases-technical note.pdfFulltext - Accepted Version340.49 kBAdobe PDFUnder Embargo until 2999-12-07    Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.