Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTaggart, Johnen_UK
dc.contributor.authorBron, Jamesen_UK
dc.contributor.authorMartin, Sam A Men_UK
dc.contributor.authorSeear, Paul Jen_UK
dc.contributor.authorHoyheim, Bjornen_UK
dc.contributor.authorTalbot, Richard Ten_UK
dc.contributor.authorCarmichael, Stephen Nen_UK
dc.contributor.authorVilleneuve, Laureen_UK
dc.contributor.authorSweeney, Glen Een_UK
dc.contributor.authorHoulihan, Dominic Fen_UK
dc.contributor.authorSecombes, Christopher Jen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorTeale, Alan Jen_UK
dc.description.abstractThe origins, design, fabrication and performance of an Atlantic salmon microarray are described. The microarray comprises 16 950 Atlantic salmon-derived cDNA features, printed in duplicate and mostly sourced from pre-existing expressed sequence tag (EST) collections [SALGENE and salmon genome project (SGP)] but also supplemented with cDNAs from suppression subtractive hybridization libraries and candidate genes involved in immune response, protein catabolism, lipid metabolism and the parr–smolt transformation. A preliminary analysis of a dietary lipid experiment identified a number of genes known to be involved in lipid metabolism. Significant fold change differences (as low as 1.2x) were apparent from the microarray analysis and were confirmed by quantitative real-time polymerase chain reaction analysis. The study also highlighted the potential for obtaining artefactual expression patterns as a result of cross-hybridization of similar transcripts. Examination of the robustness and sensitivity of the experimental design employed demonstrated the greater importance of biological replication over technical (dye flip) replication for identification of a limited number of key genes in the studied system. The TRAITS (TRanscriptome Analysis of Important Traits of Salmon)–salmon genome project microarray has been proven, in a number of studies, to be a powerful tool for the study of key traits of Atlantic salmon biology. It is now available for use by researchers in the wider scientific community.en_UK
dc.publisherWiley-Blackwell / The Fisheries Society of the British Islesen_UK
dc.relationTaggart J, Bron J, Martin SAM, Seear PJ, Hoyheim B, Talbot RT, Carmichael SN, Villeneuve L, Sweeney GE, Houlihan DF, Secombes CJ, Tocher DR & Teale AJ (2008) A description of the origins, design and performance of the TRAITS-SGP Atlantic salmon Salmo salar L. cDNA microarray. Journal of Fish Biology, 72 (9), pp. 2071-2094.;
dc.rightsRights according to Exclusive Licence Form:; Published in Journal of Fish Biology by Wiley-Blackwell / The Fisheries Society of the British Isles. Journal of Fish Biology, Volume 72, Issue 9, June 2008, pp. 2071 – 2094.; This article is the author's final, refereed version. The definitive version is available at www.blackwellsynergy.comen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectDNA microarrayen_UK
dc.subjectgene expressionen_UK
dc.subjectlipid metabolismen_UK
dc.subjectimmune responseen_UK
dc.titleA description of the origins, design and performance of the TRAITS-SGP Atlantic salmon Salmo salar L. cDNA microarrayen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[taggart et al 2008.pdf] Publisher requires a 24 month embargo period after publicationen_UK
dc.rights.embargoreason[Taggart et al TRAITS paper.pdf] Publisher requires a 24 month embargo period after publicationen_UK
dc.citation.jtitleJournal of Fish Biologyen_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Aberdeenen_UK
dc.contributor.affiliationCardiff Universityen_UK
dc.contributor.affiliationNorwegian School of Veterinary Scienceen_UK
dc.contributor.affiliationRoslin Instituteen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationCardiff Universityen_UK
dc.contributor.affiliationUniversity of Aberdeenen_UK
dc.contributor.affiliationUniversity of Aberdeenen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorTaggart, John|0000-0002-3843-9663en_UK
local.rioxx.authorBron, James|0000-0003-3544-0519en_UK
local.rioxx.authorMartin, Sam A M|en_UK
local.rioxx.authorSeear, Paul J|en_UK
local.rioxx.authorHoyheim, Bjorn|en_UK
local.rioxx.authorTalbot, Richard T|en_UK
local.rioxx.authorCarmichael, Stephen N|en_UK
local.rioxx.authorVilleneuve, Laure|en_UK
local.rioxx.authorSweeney, Glen E|en_UK
local.rioxx.authorHoulihan, Dominic F|en_UK
local.rioxx.authorSecombes, Christopher J|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorTeale, Alan J|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameTaggart et al TRAITS paper.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
taggart et al 2008.pdfFulltext - Accepted Version382.78 kBAdobe PDFView/Open
Taggart et al TRAITS paper.pdfFulltext - Accepted Version443.93 kBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.