Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24649
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dc.contributor.authorKhanam, Taslimaen_UK
dc.contributor.authorDavie, Andrewen_UK
dc.contributor.authorMcAndrew, Brendanen_UK
dc.contributor.authorPenman, Daviden_UK
dc.date.accessioned2016-12-09T03:14:50Z-
dc.date.available2016-12-09T03:14:50Z-
dc.date.issued2016-12en_UK
dc.identifier.urihttp://hdl.handle.net/1893/24649-
dc.description.abstractFirst paragraph: Genotypic information is widely used in the conservation and management of wild fish (Schwartz, Luikart & Waples 2007) and is increasingly valuable in the management of captive populations (e.g. for verification of pedigree or for marker-assisted selection; Perez-Enriquez, Takagi & Taniguchi 1999; Sonesson 2007). Obtaining DNA most commonly involves taking tissue samples from live fish, such as blood or a fin biopsy (Campanella & Smalley 2006), or killing fish (particularly if too small to take biopsy samples: Mirimin, O'Keeffe, Ruggiero, Bolton-Warberg, Vartia & Fitzgerald 2011). Tissue biopsy may have negative impacts on fish, potentially including infection and effects on survival, growth or behaviour (Le Vin, Adam, Tedder, Arnold & Mable 2011); e.g. the adipose fin, commonly removed from salmonids for marking and DNA extraction, is now known to show sexual dimorphism (Hisar, Sönmez, Hisar, Budak & Gültepe 2013) and is thought to function as a flow sensor (Buckland-Nicks, Gillis & Reimchan 2011). In studies on wild fish populations, particularly rare or uncommon species with small body size, it is undesirable to kill large numbers, and this has led to recent publications investigating extraction of DNA from mucus taken from the skin or mouth cavity (Campanella & Smalley 2006; Livia, Antonella, Hovirag, Mauro & Panara 2006; Le Vin etal. 2011; Mirimin etal. 2011; Reid, Kidd & Wilson 2012; de Verdal, Vandeputte, Pepey, Vidal & Chatain 2014). Brush swabbing is one of the less invasive sampling strategies, which is used in a range of other groups of organisms, e.g. humans (Clarke, Prost, Stanton, White, Kaplan & Matisoo-Smith 2014), amphibia (Pidancier, Miquel & Miaud 2003) and molluscs (Henley, Grobler & Neves 2006). The reduced invasiveness of such sampling techniques, and reduced likelihood of impact on the welfare of the fish concerned, also makes them less likely to come under regulations on animal experimentation; swab samples can also be collected without the use of anaesthesia, required under UK regulations for tissue biopsy (Le Vin etal. 2011). Potential drawbacks of such methods may include sample contamination, low DNA quantity or poor quality DNA (Taberlet, Waits & Luikart 1999; Lieber, Berrow, Johnston, Hall, Hall, Gubili, Sims, Jones & Noble 2013).en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationKhanam T, Davie A, McAndrew B & Penman D (2016) DNA sampling from mucus in the Nile tilapia, Oreochromis niloticus: Minimally invasive sampling for aquaculture-related genetics research. Aquaculture Research, 47 (12), pp. 4032-4037. https://doi.org/10.1111/are.12809en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectNile tilapiaen_UK
dc.subjectOreochromis niloticusen_UK
dc.subjectDNAen_UK
dc.subjectgenetic markeren_UK
dc.subjectPCRen_UK
dc.titleDNA sampling from mucus in the Nile tilapia, Oreochromis niloticus: Minimally invasive sampling for aquaculture-related genetics researchen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-13en_UK
dc.rights.embargoreason[Taslima_et_al-2016-Aquaculture_Research.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1111/are.12809en_UK
dc.citation.jtitleAquaculture Researchen_UK
dc.citation.issn1365-2109en_UK
dc.citation.issn1355-557Xen_UK
dc.citation.volume47en_UK
dc.citation.issue12en_UK
dc.citation.spage4032en_UK
dc.citation.epage4037en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaild.j.penman@stir.ac.uken_UK
dc.citation.date12/06/2015en_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000387663000033en_UK
dc.identifier.scopusid2-s2.0-84931043265en_UK
dc.identifier.wtid595578en_UK
dc.contributor.orcid0000-0002-9524-618Xen_UK
dc.contributor.orcid0000-0001-7384-5133en_UK
dc.contributor.orcid0000-0001-8608-6631en_UK
dc.date.accepted2015-05-06en_UK
dcterms.dateAccepted2015-05-06en_UK
dc.date.filedepositdate2016-12-08en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorKhanam, Taslima|en_UK
local.rioxx.authorDavie, Andrew|0000-0002-9524-618Xen_UK
local.rioxx.authorMcAndrew, Brendan|0000-0001-7384-5133en_UK
local.rioxx.authorPenman, David|0000-0001-8608-6631en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-13en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameTaslima_et_al-2016-Aquaculture_Research.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1355-557Xen_UK
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